旱稻孢囊线虫在广西的发生及其rDNA-ITS异质性分析.pdfVIP

中国水稻科学 (ChinJRiceSci)，2014，28(1)：78—84 http w w．ricesci．cn 78 DOI：10．3969／j．issn．1001—7216．2014．01．011 旱稻孢囊线虫在广西的发生及其rDNA—ITS异质性分析 卓侃1 宋汉达1 王宏洪1 陶冶 张洪玲 陆秀红2 黄金玲2 刘志明 廖金铃l (1华南农业大学 资源环境学院 植物线虫研究室，广州 510642；2广西农业科学院 植物保护研究所 ，南宁 530007； 通讯联系人， E—mail：jlliao@ scau．edu．cn) OccurenceofHeterodera elachista in GuangxiRegion and Its Intra—species HeterogeneityinrDNA—ITSRegion ZHUOKanI，SONGHan-daI，WANGHong-hong ，TAOYe ，ZHANGHong—ling ，LUXiu—hong。，HUANGJin— ling ．LIUZhi—ming。．LIAOJin—ling， (ILaboratory of PlantNematology，Collegeof NaturalResourceand Environment，SouthChinaAgriculturalUniversity， Guangzhou510642，China；2PlantProtectionResearch Institute，GuangxiAcademyofAgriculturalSciences，Nanning530007， China； Correspondingauthor，E—mail：jlliao@5cau．edu．cn) ZHUO Kan，SONG Handa，WANG Honghong，eta1．OccurenceofHeteroderaelachista inGuangxiRegionandits intra—speciesheterogeneityinrDNA_ITSregion．ChinJRiceSci，2O14，28(1)：78—84． Abstract：In2011，acystnematodewasisolatedfrom ricerootsandsoilsinLongshengTerraceofGuangxiRegion， China．MorphologicalcharacteristicsofthecystnematodewereconsistentwithpreviousdescriptionsofHeteroderaela— chista．TherDNA ITSsequencesofthecystnematodeandH ．elachista inGenBankshared97．7 一 99．3 identities． ThephylogenetictreebasedonrDNA—ITSshowedthatthecystnematodewasinthesamemonophyleticcladeasH ． Plachista with i00 supportand shortbranchdifferences．TheresultsindicatedthecystnematodewasHeterodera elachista．Therestriction enzymecutting sitesoften rDNA—ITS sequencesofH ．elachista amplified and theone reportedbyTanhaMaafi(GenBankaccessionnumber：AF498391)wereanalyzed．Therestrictionenzymecuttingsites ofAZ“I，AvaI，Mva I andRsaI inthese11sequenceswereidentica1，howevertherewere2differentpatterns