基于杜仲转录组序列的SSR分子标记的开发-林业科学.PDFVIP

第 49 卷 第 5 期 林 业 科 学 Vol. 49 ，No. 5 2 0 1 3 年 5 月 SCIENTIA SILVAE SINICAE May ，2 0 1 3 doi :10 ．11707 / j ．100 1-7488 ．20 130523 * 基 于杜仲转录组序 列 的 SSR 分子标 记 的开发 黄海 燕 杜 红岩 乌 云塔娜 刘攀 峰 (中国林业科学研究院经 济 林研究 开发 中心 国家 林业局 杜 仲 工程 技 术研究中 心 郑 州 450003 ) 关键词 : 杜 仲 ; 转 录组 ; SSR ; 引物 中图分类号 : S7 18. 46 文献标识码 : A 文章编号 : 100 1 － 7488 (20 13) 05 － 0 176 － 06 Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eucommia ulmoides Huang Haiyan Du Hongyan Wuyun Tana Liu Panfeng (Non-Timber Forest Research and Develop ment Center of Chinese A cademy of Forestry The Eucommia Eng ineering Research Center of State Forestry A dministration Zhengzhou 450003 ) Abstract : To study the genetic diversity of Eucommia ulmoides without information of the whole genome ，the SSR primers were designed based on the transcriptome sequencing data (unpublished) from leaves and fruits of E ． ulmoides． The SSR loci were analyzed using microsatellite locus scan tool SSRIT to analyze 49 6 10 sequences ，and we screened out 1 442 SSR loci which distributed in 1 334 sequences ，accounting for 2. 9 % of the transcriptome sequences． The dinucleotide repeat is the most abundant repeat type ，accounting for 69. 90 % of the total number of SSRs． We observed 150 kinds of repeating units and found that the highest frequency is AG / TC ，accounting for 32. 73 % of the total number of SSRs． Ad