大鼠超氧化物歧化酶SODELISA试剂盒说明书.PDFVIP

Rat Superoxide Dimutase(SOD) ELISA Kit Catalog No. CSB-E08555r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat SOD concentrations in serum, plasma and Tissue Homogenates. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. 1 PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with an antibody specific to SOD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SOD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3,5,5 tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve. DETECTION RANGE 6.25 mIU/ml-400 mIU/ml. The standard curve concentrations used for the ELISA’s were 400 mIU/ml, 200 mIU/ml, 100 mIU/ml, 50 mIU/ml, 25 mIU/ml, 12.5 mIU/ml, 6.25 mIU/ml. SPECIFICITY This assay recognizes rat SOD. No significant cross-reactivity or interference was observed. SENSITIVITY The minimum detectable dose of rat SOD is typically less than 1.56 mIU/ml. The sensitivity of this assay,