参芪降糖颗粒对高糖环境下施万细胞氧化应激的调节作用.PDF

摇1194 环球中医药2017年11月第10卷第11期摇 Global Traditional Chinese Medicine,November2017,Vol郾10,No郾11 ·论著 · 参芪降糖颗粒对高糖环境下施万细胞 氧化应激的调节作用 康学摇 许保海 揖摘要铱摇 目的摇 探讨参芪降糖颗粒对高糖环境下施万细胞氧化应激的调节作用。方法摇 采用 高糖环境下培养的施万细胞建立氧化应激模型,实验分为正常对照组、高糖组、参芪降糖含药血清 组和甲钴胺含药血清组,干预48 小时后,用酶标仪检测细胞中总抗氧化能力(total antioxidant capacity,T鄄AOC)、超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)含 量,应用流式细胞仪检测活性氧分子(reactive oxygen species,ROS)水平。结果摇 高糖干预48小时 后,与正常对照组比较,高糖组细胞 T鄄AOC 和 SOD 含量明显降低,ROS、MDA 水平显著升高 (P0.01);与高糖组比较,参芪降糖含药血清能显著提高细胞T鄄AOC和SOD含量,明显下调ROS 和MDA水平(P0.05)。结论摇 参芪降糖颗粒能够明显提高具有抗氧化能力物质T鄄AOC和SOD 水平,清除有害的氧化代谢产物ROS和MDA,通过抑制高糖环境下施万细胞的氧化应激而发挥对 糖尿病周围神经病变的保护作用。 揖关键词铱摇 氧化应激;摇 施万细胞;摇 糖尿病周围神经病变;摇 参芪降糖颗粒 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2017郾11郾002 Effects of Shenqi Jiangtang granule on oxidative stressin Schwann cells with high glucose environment摇 KANG Xue, XU Baohai. 摇 Pharmacy of TCM, Beijing Jishuitan Hospital, Beijing 10035,China Corresponding author:XUBaohai,E鄄mail:xubaohai70@163.com 揖Abstract铱摇 Objective摇 To explore the effects of Shenqi Jiangtang granule on oxidative stress in Schwann cellswith high glucose environment. Methods摇 Oxidative stress model of Schwann cells was es鄄 tablished byhighglucoseenvironment. Theexperimentwasdividedintonormalcontrolgroup,highglucose group,ShenqiJiangtang serum group and methylcobalamin serum group and treatment for 48 hours (h). Microplate Reader was used to detect the content of total antioxidant capacity (T鄄AOC), superoxide dismutase (SOD) and malondialdehyde (MDA). Flow cytometrywasusedto analysisthelevel of reactive oxygen species (ROS). Results摇 After incubated with high glucose for 24 h,the content of T鄄AOC and SOD in high glucose group were significantly lower,while ROS and MDA activity were significantly higher than normal control group (P0.01). Compared with high glucose group,Sh