硫化氢对白桦悬浮细胞中多胺代谢和次生代谢物积累的调控作用.DOC

硫化氢对白桦悬浮细胞中多胺和次生 代谢物积累的影响 王紫晴，李飞洁，郭玉婷，于昭慧，马钰斌，周文洋，范桂枝※ (东北林业大学生命科学学院，黑龙江省哈尔滨市，150040) 摘要：本文旨在硫化氢（H2S）将不同浓度H2S供体硫氢化钠（NaHS）添加到白桦悬浮培养体系中，采用高效液相色谱法和法多胺含量、多胺合成分解酶活性次生代谢物含量。结果表明： NaHS处理后白桦悬浮细胞中呈先增加后降低趋势，其中1mmol/L NaHS处理24h时腐胺和亚精胺总含量达最高值，分别比对照增加了30.51%和12.37%；高氯酸不溶性结合态亚精胺NaHS处理可溶性结合态NaHS处理后ADC）和PAO）活性增加幅度分别为15.91%~26.33%和137.50%~250.12%；NaHS与不同浓度的腐胺、亚精胺和精胺处理后不同程度高了白桦悬浮细胞中多酚、黄酮和三萜含量。5 mmol/L 腐胺与NaHS共同处理后多酚、黄酮和三萜含量达最大值，分别比NaHS单独处理增加了26.69%、13.22%和 4.55%。H2S处理提高了白桦悬浮细胞中多胺和次生代谢物含量，H2S与多胺共同处理可进一步促次生代谢物的累积。Tittle：The of H2S on the Accumulation of Polyamines and Secondary Metabolites in Birch Suspension Cells Abstract: The purpose of this study was to clarify the effect of hydrogen sulfide (H2S) on the accumulation of polyamines and secondary metabolites in birch suspension cells. Different concentrations of H2S donor sodium hydrosulfide (NaHS) were added to birch suspension culture, high performance liquid chromatography and colorimetric method were used to analyze the content of different forms of polyamines, the enzyme activity of arginine decarboxylase (ADC) and polyamine oxidase (PAO) as well as the content of flavonoid, polyphenol and triterpenoid. The results showed that the change of total polyamines content in birch suspension cells resembled unimodal during 6~96h after NaHS treatment. Among of them, the highest content of putrescine and spermidine was at 1 mmol/L NaHS treatment for 24h, the increasing rate was 30.51% and 12.37% comparing with that of the control, respectively; Except for perchloric acid (PCA) insoluble spermidine conjugate, NaHS treatment increased the content of free state, PCA soluble and insoluble putrescine and conjugates; Similarly, NaHS treatment enhanced the activity of ADC and PAO, the increasing rangs were 15.91%~26.33% and 137.50%~250.12%, respectively; The treatment of 1mmol/L NaHS and different concentrations of putrescine, spermidine and spermine increased the content of polyphenol, flavonoid and triterpenoid, respectively. Among of them