RP-HPLC测定延胡索酸泰妙菌素含量.PDFVIP

RP-HPLC 测定延胡索酸泰妙菌素含量 陈爱瑛1 2 1 1* ，管晨 ，徐世芳 ，姜丽霞 (1.浙江省医学科学院药物研究所，杭州 310013 ；2.浙江大学阳光营养技术有限公司, 杭州 310007) 摘要：目的 建立高效液相色谱法测定延胡索酸泰妙菌素含量。方法 采用Agilent C18 (150 mm ×4.6 mm , 5 µm), 流动相为 0.1 mol·mL-1 碳酸铵缓冲液(取碳酸铵 10 g，加水800 mL 使溶解，加24 mL 6%的高氯酸水溶液，加水至1 000 mL)-乙腈- 甲 醇(27 ∶23 ∶50)；检测波长为212 nm。结果 线性范围为0.25~0.75 mg·mL-1 (r=0.999 4)；平均回收率为99.3% (RSD=1.2%)。 结论 方法快捷，准确，重复性好。 关键词：延胡索酸泰妙菌素；高效液相色谱法；含量 中图分类号：R917.101; R927.2 文献标志码：B 文章编号：1007-7693(2009)09-0766-03 Determination the Content of Tiamulin Fumarate by RP-HPLC 1 2 1 1* CHEN Aiying , GUAN Chen ，XU Shifang ，JIANG Lixia (1.Institute of Medica Materia, Zhejiang Academy of Medicine, Hangzhou 310013, China; 2.Zhejiang University Sunny Nutrition Tec hnology Co., LTD., Hangzhou 310007, China) ABSTRACT: OBJECTIVE To establish a method for determination the content of tiamulin fumarate. METHODS RP-HPLC was adopted, the column was Agilent C18 (150 mm×4.6 mm, 5 µm), the mobile phase was 0.1 mol·mL-1 ammonium carbonate buffer (add 10 g ammonium carbonate to a 1 000 mL volumetric flask and dissolve in about 800 mL of water. Add 24 mL 6% of perchloric acid and dilute with water to volume) -acetonitril-methanol (27 ∶23 ∶50). UV dector wavelength was 212 nm. RESULTS The calibration is linear between 0.25-0.75 mg·mL-1. The mean recovery was 99.3%, RSD%=1.2%. CONCLUSION This method inspecting tiamulin fumarate is rapid, accurate and reproducible. KEY WORDS: tiamulin fumarate; HPLC; content 延胡索酸泰妙菌素是一种半发酵半合成双萜类动 物蛋白的合成，从而达到抗菌作用。主要用于抗支原 物专用抗生素。它通过干扰微生物核蛋白体抑制微生 体、螺旋体、放线杆菌病原体和赤痢菌，用于预防和 作者简介：陈爱瑛，女，高级实验师 Tel: (0571E-mail: chay-01@163.com *通信作者：姜丽霞，女，硕士