第一章分子克隆工具酶.PDFVIP

第一章 分子克隆工具酶 1 第一节 限制性内切酶 Restriction Enzymes Restriction Enzymes An endonuclease （核酸内切酶）that recognizes specific nucleotide sequences (4-8 bp) in DNA and breaks the DNA chain at that sites 2 一、限制性内切酶的发现 (The discovery of Restriction Enzymes) 1．细菌限制修饰系统 （Restriction and Modification System of Bacteria) 3 （1）限