北京鸭伊番鸭杂种优势相关基因表达差异研究.PDFVIP

南京农业大学学报摇 2010,33 (1): 113 117 摇 Journal of NanjingAgricultural University http:/ / nauxb郾njau郾edu郾cn 单雪松, 崔庆媛, 李克鹏, 等. 北京鸭伊番鸭杂种优势相关基因表达差异研究 [J]. 南京农业大学学报,2010, 33(1): 113 117 北京鸭伊番鸭杂种优势相关基因表达差异研究 单雪松, 崔庆媛, 李克鹏, 王雪丽, 杨雨江 (吉林农业大学动物科学技术学院, 吉林 长春 130118) 摘要: 用抑制性消减杂交 (SSH) 方法研究动物杂种相关基因表达差异。 选用北京鸭、 法国番鸭及其正反交F 代, 以杂 1 种组为处理, 亲本组为对照, 建立肌肉组织SSH基因文库, 随机选取1000个单克隆测序, 并与GenBank序列进行比对 分析。 结果共发现40个新ESTs,93个未知功能蛋白基因, 34 个已知功能蛋白基因。 在34 个已知功能蛋白基因中, 杂 种表达上调的基因有23个, 杂种表达下调的有11个。 研究表明杂种优势分子遗传基础复杂, 涉及能量代谢、 信号传导 及调控、 转录调控、 蛋白质合成加工等基因的差异表达。 关键词: 杂种优势; 抑制性消减杂交; 北京鸭; 番鸭 中图分类号: S813郾22摇 摇 摇 文献标志码: A摇 摇 摇 文章编号: 1000 2030 (2010) 01 0113 05 Analysis on differential expression of hybrid vigor relevant genes in domestic Beijing duck and muscovy duck crossbreeding system SHAN Xue鄄song, CUI Qing鄄yuan, LI Ke鄄peng, WANG Xue鄄li, YANG Yu鄄jiang (College of Animal Sciences and Technology, Jilin Agricultural University, Changchun 130118, China) Abstract: This experiment was conducted to study the expression difference of hybrid vigor relevant genes through the method of suppression subtractive hybridization (SSH). Beijingduck, Francemuscovyduckandthetheirmutualhybridsweredividedinto2 groups, the hybrids being the treatment and the parents being the control to construct SSHgene library. 1000 cloneswere picked randomly for sequencing. The nucleotide sequenceswere analyzed through NCBI blasting procedure. The blasting results revealed 40 new ESTs,93 hypothetical protein transcripts and34 functional protein gene, among which 23 were up鄄regulated and 11were down鄄regulated in hybrids. These results indicated that the molecular basis of hybrid vigor refers to complicated progress including energy metabolism, signal transduction, transcript regulation a