低温和湿度胁迫对盛铃期Bt棉叶片Bt蛋白表达量的影响.pdf

棉 花 学 报 Cotton Science 20 14袁26 4 冤院290~294 温和湿度胁迫对盛铃期Bt 棉叶片Bt 蛋白表达量的影响 1 1 1 1 1 1 2 1 1* 陈源 韩勇 花明明 王俊 顾超 许映飞 李国生 张祥 德华 1. 州大学农学院袁江苏扬州 225009 曰2. 州大学袁江苏扬州225009 冤 摘要院采用盆栽试验袁以转Bt 基因的常规品种DP4 10B 和泗抗1 号尧杂交种岱杂1 号和泗抗3 号为材料袁探讨 盛铃期18 低温下不同湿度对Bt 棉叶片Bt 蛋白表达量的影响遥 结果表明袁2010 年袁低温下90%尧70%尧50% 这3 种湿度胁迫6 h 后袁DP4 10B 和岱杂1 号叶片Bt 蛋白含量比各自对照 温度为常温袁湿度70% 冤显著降低袁 但泗抗1 号和泗抗3 号没有显著变化曰2011 年袁 当低温下90%尧50%湿度胁迫时间延长至48 h 后袁4 个品种叶 片中Bt 蛋白含量比各自对照均显著下降遥其中岱杂1 号和DP4 10B 在胁迫12 h 后便开始显著低于各自对照袁 而泗抗1 号和泗抗3 号则在24 h 以后才显著低于对照遥 说明 低温和湿度胁迫时间延长袁Bt 棉叶片中Bt 蛋 白含量下降效应逐渐增大袁但不同品种间存在一定差异遥 关键词院Bt 棉曰Bt 蛋白曰低温曰相对湿度 中图分类号院S562.01 文献标志码院A 文章编号院1002-7807 20 14冤04-0290-05 1 1 1 1 1 1 2 1 Zhang Xiang , Han Yong , Hua Mingming , Wang Jun , Gu Chao , Xu Yingfei , Li Guosheng , Chen Yuan , Chen Dehua1* 1. 225009, ; 2. 225009, 冤 The obj ect of this study was to investigate the effect of stresses of 18 and different relative humidity on the Bt pro- tein content in leaves at the bolling stage for Bt cotton . Two cultivars (DP4 10B and Daiza 1) from US and two cultivars (Sikang 1 and Sikang 3) from China were used as materials. The potted cotton plants were stressed at 18 and different relative humidity (50%, 70% and 90%) for 6 hours and 48 hours, respectively. The results showed that in 20 10, contrasted with control (temperature was 25-30 and humidity was 70%), the short period(6 h) stress of 18 and different relative humidity reduced the Bt protein content in leaves of DP4 10B and Daiza 1 significantly, respectively, but had no effects on th