油松成熟胚培养不定芽状态对其生根状况的影响.pdf

50 5 林 业 科 学 Vol. 50 ，No. 5 第 卷 第 期 2 0 1 4 5 SCIENTIA SILVAE SINICAE May ，2 0 1 4 年 月 doi :10 ．11707 /j ．1001-7488 油松成熟胚培养不定芽状态对其生根状况的影响* 1 2 1 2 1 买凯乐 邵砾群 李荣珍 董丽芬 兰健花 (1． 广西生态工程职业技术学院 柳州545004 ; 2 ． 西北农林科技大学 杨凌7 12100) : ; ; 1/ 2MS ; ; 关键词 油松 成熟胚 改良 培养基 不定芽诱导 不定芽生根 中图分类号:S723. 1 + 3 文献标识码:A 文章编号:1001 － 7488 (2014)05 － 0147 － 07 Effect of the Status of Adventitious Buds In Vitro Cultured from Mature Embryo of Pinus tabulaef ormis on Its Ｒooting Mai Kaile1 Shao Liqun2 Li Ｒongzhen1 Dong Lifen2 Lan Jianhua1 (1． Guangxi Eco-Engineering Vocational and Technical College Liuzhou 545004 ;2． Northwest A＆F University Yangling 7 12100) Abstract : The mature embryo of Pinus tabulaef ormis was used as explants to induce adventitious buds and root on modified 1/ 2MS medium． The results showed that the largest multiplication rate with strong buds was obtained when adventitious bud cluster was divided into three pieces． The rooting rate of adventitious buds was 91． 7 % ． The adventitious bud clusters with diameter larger than 0 ． 9 cm ，and the shoots with length longer than 6 mm had higher rooting rate． In the process of shoot elongation and multiplication ，short culture duration could lead to a higher rooting capacity of the adventitious bud cluster ，and the 28-days culture period was optimal for rooting ，leading to a rooting rate of 73． 3% ． Adventitious bud clusters with big biomass were easily to root ，and the higher the rooting rate the bigger the bud cluster biomass． Key words : Pin