通络方剂对糖尿病大鼠肝组织氧化应激水平及超微-第三军医大学学报.docVIP

通络方剂对糖尿病大鼠肝纤维化相关血清学指标及形态学的影响 沈 婕，夏新新，邹俊杰，石勇铨，刘志民 （200003 上海，第二军医大学长征医院内分泌科） 提要： 目的 探讨通络方剂对糖尿病大鼠肝纤维化相关血清学指标及形态学的影响。方法 SD大鼠予链脲佐菌素60 mg／kg腹腔注射，制备糖尿病模型。模型动物以随机数字表法分为2组（每组10只）：糖尿病组(DM组)、通络方剂干预组（TLR组），同时取10只大鼠作为正常对照组(CN组)。造模12周后测血清肝纤维化指标，取肝组织测SOD、MDA，观察肝组织形态学改变。结果 糖尿病大鼠血清HA、IV-c水平显著升高(P0．01)，肝血窦边缘及肝汇管区胶原纤维增生，Disse间隙(P0．01)。经通络方剂治疗12周后，血清HA、IV-c水平显著降低(P0．05)，肝纤维化病理形态有明显改善；糖尿病大鼠肝组织SOD活性显著增加、MDA水平显著下降(P0．01)，氧化应激水平有所下降。结论 通络方剂降低糖尿病大鼠血清肝纤维化指标、改善肝纤维化的病理形态，其作用机制部分与其增强了肝组织抗氧化酶类活性以及清除脂质过氧化物有关。 [关键词] 糖尿病；肝纤维化；通络方剂；氧化应激；超微结构 [中图法分类号] [文献标志码] A Effects of Tongluo recipe on serum hepatic fibrosis markers and hepatic morphological changes of diabetic rats Shen Jie, Xia Xinxin, Zou Junjie, Shi Yongquan, Liu Zhimin （Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China） Abstract Objective To investigate the effects of Tongluo recipe (TLR) on serum hepatic fibrosis markers and hepatic morphological changes of diabetic rats. Methods Experimental diabetes was induced by intraperitoneal injection of streptozotocin (60 mg／kg) in male Sprague-Dawley rats. The study included the following 3 groups：normal control(CN)，diabetes mellitus control(DM), TLR treatment group(TRL) , 10 rats in each group. After 12 weeks of treatment, serum hepatic fibrosis markers: hyaluronic acid(HA),collagen type IV(IV-c),laminin(LN),procollagen type III(PC III),and superoxide dismutase and malondialdehyde in liver tissues were measured．The morphological changes of liver tissues were observed through light microscope and transmission electronic microscope. Results The serum HA and Ⅳ-c levels of diabetic rats were increased (P 0.01).Light microscope and transmission electron microscope observation showed collagen fibers hyperplasia in hepatic sinusoid and portal area; Fat-storing cells hyperplasia in Disse space. The SOD activities were decreased and the MDA levels were increased in liver tissues of diabetic rats (P 0.01).After 12 wee