心肌缺血-再灌注损伤对大鼠心电图的影响-第三军医大学学报.docVIP

心肌缺血-再灌注损伤大鼠心电图变化机制的研究 王彦赵英男邹丽琳许莹平赵赫男摘　要目的探讨大鼠心肌缺血再灌注损伤过程中，氧自由基及心肌细胞凋亡与缺血再灌注性心律失常的关系。方法建立鼠心肌缺血-再灌注模型，对比缺血期与再灌注期心律失常发生率及平均持续时间、ST段改变、心肌丙二醛含量及超氧化物歧化酶活力、心肌细胞凋亡情况。结果⑴缺血期房室传导阻滞、室性早搏及室速的发生率分别为7.5%、15%、10%；再灌注后上述指标分别为30%、67.5%及32.5%。与缺血期相比，再灌注后上述指标均显著增高。室速的平均发作时间由缺血期的40±14 s上升到495±103 s（P0.01）；再灌注后室颤平均持续时间为198±22 s。⑵再灌注后心肌MDA含量高于缺血组（P0.01），且逐渐递增趋势；心肌SOD活力较缺血组降低（P0.01），再灌注120 min后下降趋缓；⑶再灌注组心肌细胞凋亡明显高于缺血组（P0.05）⑷缺血再灌注损伤过程中，心律失常发生率与SOD活力变化呈负相关，与MDA值变化及心肌细胞凋亡变化均成正相关。结论氧自由基及心肌细胞凋亡很可能是造成心肌缺血再灌注后心律失常的重要因素关键词心肌缺血再灌注；心肌凋亡；心电图；氧自由基；再灌注性心律失常 The study on mechanism of ECG change during myocardial ischemia-reperfusion process WANG Yan1, ZHAO Ying-nan2, ZOU Li-lin3, XU Ying-ping3, ZHAO He-nan4 (Dalian Medical University: 1Laboratory center, the second Affiliated Hospital of, Dalian 116023, 37-year Program Student of Grade 2005, 4Department of Pathophysiology; 2Center for Disease Control of Heilongjiang province, Harbin 150023, Heilongjiang province, China) Abstract: Objective To investigate the relationship between oxygen free radicals and myocardial apoptosis and arrhythmia during myocardial ischemia-reperfusion injuries. Methods Duplicated myocardial ischemia-reperfusion models of rats, and analyzed incidence rate and duration of arrhythmias, changes of ST segment, MDA value, the activity of SOD and the state of myocardial apoptosis between ischemic and reperfusion process. Results ⑴ The incidences of atrioventricular block，ventricular premature beat and ventricular tachycardia were 7.5%,15% and 10% respectively. After reperfusion, the above indicators increased to 30%, 67.5% and 32.5% respectively. Compared to ischemia period, they all increased significantly. The duration of the ventricular tachycardia increased form 40±14 s to 495±103 s after reperfusion. During the ischemia period, the duration of ventricle fibrillation in reperfusion was 198±22 s. ⑵ The content of MDA in the reperfusion group is more than that in ischemia group（P0.01）, and revealed the increasing trend after reper