莲子心生物碱树脂法分离纯化工艺研究.PDFVIP

Drug Evaluation Research 第 36 卷 第 3 期 2013 年 6 月 - 199 - 莲子心生物碱树脂法分离纯化工艺研究 吴梅青，陈 丹 湘潭职业技术学院，湖南 湘潭 411102 摘 要：目的 筛选适用于分离纯化莲子心生物碱的大孔吸附树脂。方法 考察吸附性能较好的 D101 、AB-8 、DA201 3 种 大孔吸附树脂对莲心碱的吸附能力及洗脱参数，并用 HPLC 定量分析了莲心碱的量。结果 D101 树脂对莲子心中生物碱有 较好的吸附分离效果。先用水洗除杂，再用 6 倍树脂体积、乙醇体积分数为 80%、pH 3 的洗脱剂洗脱，浓缩干燥，产品中 莲心碱的质量分数为 10.2%、产品收率为24.6% 。结论 该工艺简单可行，分离效果好，适合莲子心生物碱的分离纯化。 关键词：莲子心；生物碱；莲心碱；分离；纯化 中图分类号：R284.2 文献标志码：A 文章编号：1674 - 6376 (2013) 03 - 0199 - 04 DOI: 10.7501/j.issn.1674-6376.2013.03.011 Separation and purification of alkaloids in Nelumbinis Plumula by resins WU Mei-qing, CHEN Dan Xiangtan Vacational and Technical College, Xiangtan 411102, China Abstract: Objective To screen the macroporous resin for the separation and purification of alkaloids from Nelumbinis Plumula . Methods The resins such as D101, AB-8, and DA201 were studied for their adsorption capability and elution parameters. The contents of liensinine were determined by HPLC. Results D101 resin had a preferable adsorptive and separative effect for the alkaloids in Nelumbinis Plumula . The preferable desorption ratio could be obtained by using 80% ethanol (pH 3) as eluting solvent and 6 BV. Under these conditions, the content of alkaloids in Nelumbinis Plumula was 10.8% and the yield was 2.6%. Conclusion The method is easy and possesses the popularization potential and good efficacy. It could be used for the separation and purification of alkaloids in Nelumbinis Plumula . Key words: Nelumbinis Plumula ; alkaloids; liensinine; separation; purification 莲子心为睡莲科植物 Nelumbo nucifera Gaertn. 高效液相色谱仪（岛津 LC—10ATVP）；岛津 的成熟种子中的干燥幼叶及胚根。主产于湖南、湖 SPD210Avp 紫外可见检测器；赛多利斯 BP211D 分 北、福建、江苏、浙江等地，具有清心安神、交通 析天平（十万分之一）；莲子心购自超市，产地为湖 心肾、涩精止血的功效。用于热入心包，神昏谵语， 南湘潭，经