蒿属花粉抗原对扁桃体淋巴细胞因子及细胞增殖的影响.pdfVIP

2004 44 4 A cta Academia e Medicinae Jiangxi 2004 Vol44 No4 1 1 1 2 3 , , , ( 1. 深圳大学生命科学学院, 广东 深圳518060 ; 2. 深圳市第一人民医院耳鼻咽喉科, 广东 深圳518020; 3. 深圳市红会医院耳鼻咽喉科, 广东 深圳518029) : , 1640 ; , ( ELISA) IL4IL5IFN, MTT IL4IL5 , IFN, ( P 0. 0 1) , ( P 0 . 01) , ( P 0 . 05) , Th2 , Th1 ,T h1 Th2 : ; ; ; ; MTT : R592 . 12 : A : 1000 - 2294 ( 2004) 04- 0001- 03 The Dynamic Changes of Cytokines Produced by Tonsil Lymphocytes after Stimulated by Artemisia Pollen Antigen 1 1 2 3 QIU Jin LIU Zhigang LIU Ming LU Yongtian ( 1. College of Lfi e Sciences, Shenzhen University , Shenzhen 518060, China; 2. Dep artment of Otolaryngology , Shenzhen Peop le s Hosp ital, Shenzhen 518020, China; 3. Dep artment of Otolaryngology , Shenzhen Real Cross H osp ital, Shenzhen 518020, China ABSTRACT: Objective To nvest gate the prol ferat on and the dynam c changes of cytok nes n tons l lym phocytes n Artem s a pollenatop c people and nonatop c people after st mulated by the spec f c ant gen. Meth ods Tons l lymphocytes were collected by lymphocytes separat on med um and ncubated w th Artem s a pollen ant gen .The levels of IL4, IL5, IFNwere measured by enzyme l nked mmunosorbent assay ( ELISA) . The prol ferat on of lymphocytes were detected w th MTT method. Results The levels of IL4, IL5 n tons l lym phocytes n Artem s a pollenatop c people after st mulated by spec f c ant gen were h gher than nonatop c con trols , but the levels of IFNwere lower than those n nonatop c controls( P 0. 01,