黑灵芝多糖的抗肿瘤活性及其分子机制初探Antitumor activity and molecular mechanism of Polysaccharide from Ganoderma lucidum.pdf
黑灵芝多糖的抗肿瘤活性及其分子机制初探Antitumor activity and molecular mechanism of Polysaccharide from Ganoderma lucidum
万方数据
万方数据
摘要
摘 要
黑灵芝多糖PSG-1 是从黑灵芝子实体中提取的多糖组分,主要由木糖、
岩藻糖、甘露糖、半乳糖、葡萄糖等组成。大量研究表明,该多糖具有抗氧
化、提高免疫力、心肌保护、抗糖尿病、抗肿瘤、抗衰老等作用,但其抗肿
瘤作用机制方面的研究甚少,本研究旨在探讨PSG-1 的抗肿瘤作用及其分子
机制,从而为其开发为一种新型药品或功能食品提供有价值的理论依据。
本研究分别从体外和体内两种途径研究PSG-1 的抗肿瘤作用及机制。本
课题在现代分子生物学理论及技术的指导下,应用流式细胞术(Flow
Cytometry, FCM) 、蛋白印迹(Western Blot) 、酶联免疫(Enzyme Linked
Immunosorbent Assay, ELISA) 及逆转录- 聚合酶链式反应(Reverse
Transcription-Polym
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