固相萃取-高效液相色谱法测定烟叶中的吡蚜酮残留量 Determination of Pymetrozine Residue in Tobacco Leaves with SPE and HPLC.pdfVIP

Science＆Technology烟草化学／TobaccoChemistry 烟草科技／Tobacco 固相萃取一高效液相色谱法测定烟叶中的“吡蚜酮残留量 单菊菊。程新胜，黄兰 中国科学技术大学烟草与健康研究中心，合肥市徽州大道1129号230052 关键词：“吡蚜酮”；烟叶；残留量；固相萃取；HPLC Silica 摘要：通过比较Sep．PakFlorisil，Sep．PakGel和Pesticarb／NH2小柱对烟叶样品萃取液的净化效果， 建立了乙腈超声提取、Pesticarb／NH，柱固相萃取净化、高效液相色谱法测定烟叶中“吡蚜酮”残留量的 方法。结果表明：“吡蚜酮”的检测限为0．005斗g／mL，平均回收率为98．1％一99．2％，相对标准偏差为 1．39％一3．06％。该方法可用于批量烟叶样品中“吡蚜酮”残留的快速测定。 中图分类号：$481+．8文献标识码：B 文章编号：1002—0861(2010)05—0038—05 of ResidueiuTobaccoLeaveswithSPEandHPLC Determination Pymetrozine SHAN HUANGLAN JU—JU，CHENGXIN—SHENG，and ResearchCenterofTobaccoand ofScienceand of China，Hefei Health，UniversityTechnology 230052，China Keywords：Pymetrozine；Tobaccoleaf；Residue；Solid-phase liquid chromatography(HPLC) for silica theeffectof tobaccoextractionwith Sep-Pak Abstract：Bycomparing purification Florisil，Sep—Pak for residuesin tobaccowas and method flue—cured gel Pestiearb／NH2cartridges，a determiningpymetrozine with and wasextractedacetonitrileunder extracted developed．Tobaccosample ultrasonic，solidphase purified witha anddetermined Pesticarb／NH2cartridge byhigh-performanceliquid resultsshowedthatthe recoveriesof from98．1％to99．2％with1．39％to average pymetrozineranged 3．06％ofrelativestandarddeviationsand oflimitofdetection．Thismethodis to 0．005恤g／mL applicable of residueinthebatchoftobaccoleaf rapidanalysispymetrozine