Platelet Rich Plasma Soft Tissue Opportunities：富血小板血浆的软组织的机会.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * PRP Update By Kenneth A. Jurist, M.D. What is PRP? PRP is simply whole blood that is centrifuged to create an increased concentration of platelets with or without WBC’s What’s so special about Platelets? Platelets contain granules that store large numbers of “growth factors” Alpha granules released (activated) when platelets aggregate and adhere to a site of injury or inflammation Listing of other growth factors: interleukin-1, tumor necrosis factor, transforming growth factor, insulin growth factor and more It is the activation of platelets and release of the various growth factors that makes them special and is the key to enhancing tissue repair and healing! Platelet Rich Plasma Biology What do these Growth Factors Do?? Chemotaxis Directional movement in response to a chemical stimulus Stems cells are attracted to the growth factors and migrate into the area Cell proliferation Significant increase in cellular reproduction activity Possibly even a systemc effect Performance enhancing? The Primary Growth Factors Role in Soft Tissue Healing and Repair PDGF 2 polypeptide chains AB, BB and AA types (AB in human platelets) Stored in alpha granules in platelets Receptors are found in tendon, cartilage, synovium and elsewhere TGF beta Also polypeptide, multiple chains, TGF beta-1 most studied form Found in platelets and bone, cartilage Also found in macrophages Receptors are universal in cells throughout the body Effect of PDGF on Tissues Immediate (within 5 minutes) Second messenger stimulation Inflammatory response Early (30min to 4 hours) M-RNA stimulation, protein synthesis Chemotaxis (draws cells to the area) Late (4-24 hours) Fibroblast mitosis Effect of TGF-Beta on Tissues Actually inhibits cell growth More of a modulator of cell growth, differentiation Very important in wound repair Summary of Effect of Platelet Growth Factors on Tissues It appears that through a complex series of biochemic