马铃薯晚疫病生防菌株的筛选及其拮抗作用的 研究.pdfVIP

Advances in Microbiology 微生物前沿, 2015, 4(4), 55-61 Published Online December 2015 in Hans. /journal/amb /10.12677/amb.2015.44008 The Study of Screening and Antagonism for Potato Late Blight Biocontrol Xu Cao1,2,3, Yushuai Liu1,2,3, Jingyu Chen2,3, Shumei Zhang1,2,3, Liqiang Meng1,2,3, Jihua Hu2,3, Wei Jiang1,2,3, Jing Li1,2,3* 1 Institute of Microbiology of Heilongjiang Academy of Sciences, Harbin Heilongjiang 2 Heilongjiang Key Lab of Biological Engineering, Harbin Heilongjiang 3 Institute of High Technology, Heilongjiang Academy of Sciences, Harbin Heilongjiang th th th Received: Nov. 20 , 2015; accepted: Dec. 6 , 2015; published: Dec. 9 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Aim: To screen biocontrol strains that with better control effect on potato late blight and then study the biocontrol strains antagonistic effect. Methods: Screening of biocontrol bacteria cultured by plate confrontation inhibition, the mechanism of antagonism was preliminarily explored by using gradient dilution method and co-culture method. Results: Screening 2 strains which had inhibitory effect on potato late blight pathogen biocontrol strains of PB12 and CZS, the antibacterial test showed that the inhibition rate of PB12 and CZS was 98%; and the change of biocontrol dilution degree in liquid culture had little effect on the antibacterial effect, while decreased slightly in the solid medium. Conclusion: We screen 2 strains with strong antagonistic effect on potato late blight pathogen biocontrol strains of PB12 and CZS, and achieve the antibacterial effect