大鼠溃疡性结肠炎肝郁脾虚证模型的研究-中国科技论文在线.pdfVIP

北京中医药大学学报 JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE 1999年 第2期 No.2 1999 大鼠溃疡性结肠炎肝郁脾虚证模型的研究* 顾立刚 郭学志 王庆国 摘 要：大鼠每天经一定时间限制活动和束绑后肢3周后，用5%醋酸灌注大鼠结 肠造成溃疡性结肠炎肝郁脾虚证模型。大鼠分别在3、6、12d后处死检测实验指标。 结果表明：模型组大鼠体重下降、粪便溏泄、肾上腺重量增加、胸腺重量下降；结 肠粘膜在光镜下可见到大量炎细胞浸润、出血、水肿、粘膜溃疡等；大鼠脾T、B淋 巴细胞增殖反应能力明显下降；全血粘度在45、18、11.25s－1切变率时比明显升 高；造模12d后，模型组大鼠下丘脑单胺类激素如去甲肾上腺素、多巴胺、5-羟色胺 显著升高；以上各项均比单纯醋酸诱导组改变明显。用疏肝健脾方药治疗后上述指 标改善明显。 关键词：肝郁；溃疡性结肠炎；肝郁脾虚证；大鼠模型；疏肝健脾 Study On Rat Model of Ulcerative Colitis With the TCM Syndrome of “Depression of Liver-energy and Asthenia of Spleen” Gu Ligang (顾立刚),Guo Xuezhi (郭学志),Wang qingguo (王庆国) (Beijing University of Traditional Chinese Medicine, Beijing 100029) ABSTRACT: Being enraged for 3 weeks and then coloclyster with 5% acetic acid, a rat model of ulcerative colitis with the “Depression of Liver-ene rgy and Asthenia of Spleen” was created. Put the mice to death respectively aft er 3、6、12 days and check the experimental index. The results show that: The bo dy weight decreased with loose stool, the weight of adrenal body increased while that of thymus gland decreased; By the light-microscope the colonic mucosa was shown inflammatory infiltration, hemorrhage, edema and mucosal ulceration, etc. The multiplicative reaction of T, B lymphocytes was remarkably -1 -1 -1 decreased and th e blood viscosity (map.s) (45s , 18s and 11.25s ) level we re significantly increase. After 1 2 days, the hypothalamic, monoamine hormone, like norepinephrine, dopamine and 5 - hydroxytryptamine, were remarkably increased. The changes of all the indexes a bove were more