河南省不同地区棉花黄萎病菌分离物致病性及其毒素致萎活性.PDFVIP

棉花 学 报 Cotton Science 2010 22 渊4 冤院333~338 南省不同地区棉花黄萎病菌分离物致病性及其毒素致萎活性测定 桑茜 袁虹霞 王振跃 李洪连 汪 敏* ( 南农业大学植物保护学院, 南郑州450002 ) 摘要院在河南省不同地区分离并鉴定33 株棉花黄萎病菌分离物 并进行了致病力和粗毒素致萎活性测定遥 致 病力结果表明 南省棉花黄萎病菌分离物可分为强尧中尧弱3 种致病力类型 分别占48.5 % 尧2 1.2 % 尧30.3 % 遥 河南省同一地区内不同分离物间致病力差异明显遥 粗毒素致萎活性测定结果表明 黄萎病菌强致病力分离物 分泌的粗毒素对棉苗的致萎力较强 弱致病力分离物分泌的粗毒素对棉苗的致萎力较弱 强尧中尧弱3 种致病 力类型分离物在72 h 时对棉苗的平均致萎指数分别为63.82尧52.38尧45.83遥 采用考马斯亮蓝法测定不同分离 物粗蛋白含量 结果表明 不同黄萎病菌分离物分泌的粗蛋白含量存在明显差异 且与其所致的病情指数呈显 著正相关遥 关键词院棉花黄萎病曰大丽轮枝菌曰致病力曰毒素 中图分类号院S435.621 文献标识码院A 文章编号院1002-7807 渊20 10冤04-0333-06 SANG Xi, YUAN Hong-xia, WANG Zhen-yue, LI Hong-lian, WANG Min* ( 450002, ) In order to research pathogenicity differentiation of on cotton in Henan, 33 isolates were isolated and identified from the main cotton-producing regions in Henan Province. The results of pathogenicity tests on cotton seedings indicated that the isolates from cotton in Henan could be divided into three pathotypes: the highly aggressive isolates, the moderate aggressive ones and the weakly aggressive ones, accounting for 48.5% , 21.2% and 30.3%, respectively. The results also showed that the pathogenicity of isolates had no significant difference among different re- gions, but the pathogenicity of isolates from the same region showed significant difference. The wilting capability of the crude toxin secreted by isolates were also tested, the results showed that the crude toxin of different isolates had different wilting capability; the crude toxin secreted by the highly aggressive isolates had strong wilting capability. The average wilt index of the highly aggressive isolates, moderate aggressive ones and weakly aggressive ones at 72 h were 63.82, 52.38 and 45.83 re- spectively at