不同月龄的SD大鼠制备心肺复苏模型后神经功能及亚组评分的比较研究.docVIP

2月龄和4月龄大鼠制备心肺复苏模型后神经功能评价及亚组评分的比较研究 田欣，王晶，赵芯晨，周小超，闫峰，何婧瑜，郭德玉 （首都医科大学宣武医院，北京100053） 摘要：目的 本研究旨在选择适合的大鼠，对比2月龄大鼠与4月龄大鼠CPR后神经功能评分及亚组评分，摸索利用不同月龄的大鼠CPR的可行性。方法 通过严格监测SD大鼠制备模型过程中的心电、血压生理指标、测定各组大鼠不同时间点的神经功能缺失评分以及神经功能亚组评分、对比制备4月龄和2月龄大鼠心肺复苏模型的稳定性。 结果 电刺激致室颤，4月龄组大鼠的成模率为87.5%，远高于2月龄组大鼠，而死亡率无差异；在电刺激诱发CA过程中造成的血压变化，4月龄组大鼠明显低于2月龄组大鼠，存在极显著性差异（P0.01）；4月龄组大鼠与2月龄组大鼠CPR后各时间点的神经功能评分无统计学差异，而4月龄组大鼠与2月龄组大鼠的神经功能亚组评分在不同时间点，存在显著差异（P0.05），4月龄大鼠与2月龄大鼠对比，心肺复苏后脑损伤的程度加重。结论 4月龄大鼠更适合制备心肺复苏模型，该月龄组的大鼠制备模型成模率高，脑损伤程度重，更适合于用于心肺复苏的基础研究及治疗评价。 关键词：心脏骤停;心肺复苏;神经功能;低灌注;脑损伤;大鼠 The Comparative Study of Neurological Function Score and Subgroups analysis for rats at different ages after CPR modeling Tian Xin，Wang Jing，Zhao Xin-chen,Zhou Xiao-chao, Yan Feng,He Jing-yu, Guo De-yu (Xuan Wu Hospital of Capital University of Medical Sciences, Beijing, 100053,China) Objective：The purpose of this study is to?select suitable ages of ?rats for the CPR (cardiopulmonary resuscitation) animal model.?The neurological function score and subgroups analysis are evaluated in 2 month old and 4 month old animal groups. Methods Based on the evaluation of physiological indexes including ECG, blood pressure and neurological function defect score (NDS) and subgroup analysis, the stability of CPR rats model was compared between 2 month old and 4 month old animal groups. Results The results showed that, the model rate of the ventricular fibrillation was induced by electrical stimulation , the 4 month old group was 87.5%, significantly higher than the 2 month old group, however, there was no significant difference between the two groups in the mortality rate; For the changes of blood pressure during the process of CA(cardiac arrest) induced by electrical stimulation, the 4 month old group was significantly lower than the 2 month old group (P0.01); for the NDS at each time point after CPR, there was no significant difference between the two groups; however, the NDS subgroup analysis at different time points showed that there were differen