海藻色素糖蛋白对肝癌细胞Ca spase-3和Bax蛋白表达的影响(英文).pdfVIP

1771 现代生物医学进展 Progress in Modern Biomedicine Vol.11 NO.9 MAY.2011 · · Effects of Seaweed Pigment Glycoprotein on Caspase-3 and Bax Protein Expression of Hepatoma Cells △ YANG Qing, ZHONG Jin -yi , LI Shuai, QUAN Xiang -hua Dep artment of Nutrition Qingdao University Medical College Qingdao 26602 1 China （ ， ， ， ） ABSTRACT Obj ective: To investigate the effect of seaweed pigment glycoprotein (SPG) on the expression of Caspase-3 and Bax protein in hepatoma cells. Methods: Fifty mice inoculated with hepatoma H22 cells were divided into five groups, 10 mice per group. The high, medium and low SPG group were administered by gastric perfusion with 100, 50 and 10 mg/kg/day SPG, respectively. The tumor control group was given the same amount of saline daily. The cyclophosphamide group was given 20 mg/kg cyclophosphamide by intraperitoneal injection every other day. The proliferation activity of hepatoma cells was determined by MTT assay. The expression of Caspase-3 and Bax protein were detected by immunohistochemistry method. Results: The expression of Caspase-3 and Bax protein in high-dose group were 40.20 % and 38.10 % , respectively, while those in the tumor control group were 5.00 % and 4.68 % . The differences between two groups were significant (p ＜0.05). The proliferation activity of high-dose group and tumor control group were 0.711±0.028 and 1.135±0.032.The difference was significant (p ＜0.05)