红花黄色素对人肝癌mhcc-97h细胞周期、凋亡及bax、bcl-2蛋白表达影响safflower yellow pigment to human liver mhcc - 97 - h cell cycle, apoptosis and bax, bcl - 2 protein expression.pdfVIP

Abstract ABSTRACT BackgroundandPurpose： Inrecentyears, duetothe increasingincidence ofcancer,adjuvanttreatment of traditional Chinese medicine in cancer also plays a significant role. Red flower in clinical medicine often used in anti-cancer compound, which was first seen in medication record Golden Chamber. Safflower Compositae safflower (Carthamus tinctorius L.) tubular corolla, acrid, warm, heart, liver, there is blood circulation, blood stasis and analgesic efficacy. Safflower ingredients are many and complex, a largenumber of studieshave shownthat saffloryellow (SY)isthe material basis of its pharmacological effects to play, more research on SY role in cardiovascular, relativelylittleresearchinoncology .HCCisthemostcommonmalignanttumorsof the digestive system, but there is a certain correlation between the development of hepatitisBvirusinfectionandlivercancer.HumanhepatomacelllineMHCC-97His thehepatitisBvirussurfaceantigenexpressionandAFPwerepositive,andhavehigh metastatic potential of cell lines, as research associated hepatocellular SYeffect on tumor cellsinfectedwiththehepatitis Bvirus isvery appropriate.Theimpact ofthe experiment SY different concentrations of MHCC-97H cell cycle, apoptosis and protein Bax, Bcl-2 expression in order to provide reference for theoretical research andclinicalapplicationofanti-tumoradjuvanttherapy. Researc Methods: MHCC-97H cells cultured in vitro in logrithmic growth phase wered selected. Then diffrenent concentrations (0,0.03125,0.0625,0.125,0.25,0.5mg/L) of SY were added to cell culture medium in matched experimental groups,and positive control grouptoCisplatin(atconcentrationof0.5μg/ml).Respectivelyinterveneof24h,48h and after 72h,morphological changeswere observedunder amicroscope, inhibition on cell proliferation of eperimental groups was evaluated by four methyl thiazolyl tetrazolium (MTT) method. The chang