刺络放血治疗膝关节骨性关节炎模型兔的实验研究兔的实验研究.pdfVIP

摘 要 目的：观察刺络放血治疗膝关节骨性关节炎（osteoarthritis of knee，KOA） 模型兔，通过实验研究解释刺络放血治疗 KOA 的可能作用机理，为临床刺络放血治 疗KOA 进一步提供科学依据。 方法：将 32 只新西兰大白兔，随机分为4 组，即空白组（A 组）、模型组（B 组）、刺络放血组（C 组）和药物治疗组（D 组），每组8 只。将A 组的兔子正常饲 养，不作任何处理；对B 组的兔子只造模；对C 组的兔子造模成功后进行刺络放血 治疗，每周两次；对D 组的兔子造模成功后每天给予药物治疗，共治疗8 周。治疗 结束后，检测其骨内压数值、血清中IL-lβ、TNF- α的含量。 结果：.B 组与A 组比较，B 组骨内压高于A 组，统计有显著性差异（p＜0.01）， 说明造模成功；C 组及D 组与B 组比较，骨内压明显降低，统计有显著性差异（右 侧p＜0.01)，C 组与D 组比较差异无统计学意义（p0.05），说明刺络放血疗法和 该药物均可有效降低骨内压，其治疗效果相当。.B 组与A 组相比，血清中IL-lβ、 TNF- α含量显著升高（p＜0.01）；C 组和D 组与B 组比较，血清中IL-1β含量显著 降低，统计有显著性差异（p＜0.01），血清中 TNF- α含量明显降低，统计有差异 （p＜0.05），C 组与D 组比较差异无统计学意义（p0.05），说明刺络放血疗法和 该药物均可降低血清中细胞因子IL-1β、TNF- α含量。 结论：刺络放血疗法能有效降低骨内压，进一步研究证明刺络放血疗法能降低血 清中IL-lβ、TNF- α含量。这些可能是刺络放血疗法治疗KOA的作用机制之一。 关键词：刺络放血疗法；KOA；骨内压；IL-lβ；TNF- α； Abstract Experimental Study of Osteoarthritis Rabbits Models Treated by Bloodletting Therapy Objective: To observe the osteoarthritis of knee (KOA ）rabbit modles treated by blood-letting puncture therapy and explain the possible mechanism in treating KOA through experimental study, providing scientific evidence that can be used to treat KOA clinically. Methods: 32 New Zealand rabbits were randomly divided into four groups: the blank group(group A),the model group(group B),the blood-letting group(group C) and the drug group(group D),eight rabbits in each group. In group A,the rabbits were fed normally without doing other managements.In group B, rabbits were built into models.In group C,the rabbits were treated by blood-letting therapy after having been moulded successfully, twice per week.In group D,after having been moulded successfully, the rabbits were given medication every day,the total treatment lasting for eight weeks. Then, detect the intraosseous pressure value and the volume of IL-lβ and TNF-α in the serum. Results: Compared with group A, the intraosseo