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本科论文-细胞工程3000字翻译
英文文献翻译
学院名称: 电气信息工程学院
专 业: 电子信息工程
班 级: 14电子1B
学 号: 201496111
姓 名: 李鹏
指导教师姓名:
指导教师职称:
二〇一六 年 六 月
Part1:cell engineering
In 2007, Li et al. [94] proposed their microfluidic device for single cell analysis. The chip was fabricated by standard 1-photomask with low cost method. The device consists four reservoirs, four channels and one open region which contain a cell retention chamber. Reservoir one was used for cell introduction and washing, whereas reservoir two was used for reagent delivery. Reservoir three and four were used as waste reservoirs. By using this chip, they quantified dynamic Ca2+ mobilization of a single cardiomyocyte during its spontaneous contraction. Also, they monitored successfully dynamic responses from various external stimulation such as daunorubicin (cardiotoxic chemotherapeutic drug), caffeine, and isoliquiritigenin (herbal anticancer). Their results also prove that anticancer drugs have less effect on the Ca2+ of the cardiomyocytes. This device has quantified the cellular response of single cardiomyocytes, discovery of heart diseases drug and cardiotoxicity testing.
In 2010, Mellors et al. [95] proposed an electrophoretic and electrospray ionization based microfluidic device for single cell analysis. The device was fabricated on corning borosilicate glass substrate by using standard photolithography and wet chemical etching technique. Figure 12 shows the schematic of the microfluidic device, where A was a cell loading reservoir and B was buffer loading, which intersects with the separation channel
This intersection zone was a cell lysis zone. CS was an electro-osmotic pump which was connected with an electrophoretic separation channel and electrospray orifice. Cells can flow throughhydrodynamically or electrically to the intersection zone, where cells were e
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