杏仁超临界CO2萃取物抗UVA对成纤维细胞的-中国现代应用药学.PDFVIP

杏仁超临界CO2 萃取物抗UVA 对成纤维细胞的损伤作用 * 陈巧云，王业秋 ，张宁，任燕冬，井丽巍(黑龙江中医药大学，黑龙江 佳木斯 154007) 摘要：目的 探讨杏仁超临界 CO2 萃取物对UVA 诱导的人皮肤成纤维细胞(ESF-1)损伤的保护作用及其作用机制。方法 用20 J·cm2 的UVA 照射ESF-1 细胞建立光老化细胞模型，以不同浓度杏仁超临界CO2 萃取物处理光老化细胞，MTT 法 检测细胞活力，羟胺法检测细胞SOD 活性，比色法检测GSH-Px 活性，TBA 法检测MDA 含量，酶联免疫法检测TNF-α、 IL-1β分泌量。结果 UVA 照射ESF-1 细胞后，细胞活力、SOD、GSH-Px 活性降低，MDA 含量升高，TNF-α、IL-1β分 泌量增加(P0.01) ，40 µg·mL1 和80 µg·mL1 杏仁超临界CO2 萃取物能显著提高成纤维细胞活力、SOD、GSH-Px 活性， 降低MDA 含量，减少TNF-α、IL-1β分泌(P0.05 或P0.01) 。结论 杏仁超临界CO2 萃取物能减轻UVA 对人皮肤成纤 维细胞的损伤，其机制可能与其清除氧自由基，抗脂质过氧化，抑制氧化损伤，减少炎症细胞因子分泌有关。 关键词：杏仁；超临界CO2 萃取；成纤维细胞；光老化 中图分类号：R965 文献标志码：A 文章编号：1007-7693(2012)09-0778-04 Protective Effects of Supercritical CO Extracts of Armeniacae Semen Amarum on Human Fibroblast 2 Damaged by Ultraviolet A * CHEN Qiaoyun, WANG Yeqiu , ZHANG Ning, REN Yandong, JING Liwei(Heilongjiang University of Chinese Medicine, Jiamusi 154007, China) ABSTRACT: OBJECTIVE To observe the protective of supercritical CO extracts of Armeniacae Semen Amarum on human 2 fibroblasts iniured by irradiation of ultraviolet A and explore its possible mechanism. METHODS The model of photoaging fibroblasts was established by irradiating of ultraviolet light(20 J·cm2). The different concentration of supercritical CO extracts 2 of Armeniacae Semen Amarum were used for photoaging cell. The cell viability, SOD activity, GSH-Px activity, MDA content were assayed by MTT, hydroxylamine, colorimetric and TBA methods, respectively. The secretion levels of TNF-α and IL-1β of cultured ESF-1 cells were detected by ELISA. RESULTS After the UVA irradiation, the cell viability, the activities of SOD and GSH-Px were decreased, the