溶液热历史对蛋白质晶体生长影响的内在原因研究StudyofIntrinsic.PDFVIP

2007 年第65 卷 化 学 学 报 Vol. 65, 2007 第13 期, 1202～1206 ACTA CHIMICA SINICA No. 13, 1202～1206 ·研究论文· 溶液热历史对蛋白质晶体生长影响的内在原因研究 戴国亮* 解 莹 康 琦 胡文瑞 ( 中国科学院力学研究所国家微重力实验室 北京 100080) 摘要 从溶液中聚集体的角度研究了溶液的热历史改变生长出的蛋白质晶体的数目和尺寸的内在原因. 将在 281 和 309 K 下保存1 d 的两组溶菌酶溶液按不同比例混合, 加入沉淀剂生长晶体. 随着高温溶液的比例增加, 生长出的晶体 数目减少, 同时溶液中生长基元的尺寸增大. 在5 周内, 采用动态光散射对281, 293 和309 K 三种温度下保存的溶菌酶 溶液中聚集体的变化情况进行监测, 发现溶液中均存在大小不同的两部分聚集体, 称之为小聚集体与多聚体. 前者的 尺寸基本不随保存时间而变化, 而后者尺寸随保存时间增加而减小, 减小的速度与保存温度有关. 多聚体的尺寸经过5 周后和小聚集体基本相同. 研究结果表明, 处于无序聚集阶段的溶液的均一化程度和成核阶段生长基元的尺寸受到了 溶液热历史的影响, 并最终对晶体的数目产生影响. 关键词 热历史效应; 溶菌酶; 聚集体; 光散射 Study of Intrinsic Reason of Effect of Solution Thermal History on Protein Crystal Growth DAI, Guo-Liang* XIE, Ying KANG, Qi HU, Wen-Rui (National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080) Abstract In this paper, the aggregates in protein solution were investigated in order to study the change inside bulk solution that affect the amount and size of protein crystal after suffering thermal history effect. Two groups of lysozyme solution stored at 281 and 309 K separately were mixed with different ratio and precipitation agent was added. It was found that the amount of crystals decreased as the increase of the ratio of the heated solution, and the size of the growth units in solution increased at the same time. The situations of aggregates in lysozyme bulk solution stored a