纤维素酶与木质纤维素生物降解转化的研究进展-中国科技论文在线.PDFVIP

中国科技论文在线 生 物 工 程 学 报 Chin J Biotech 2010, July 25; 26(7): 864–869 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2010 CJB, All rights reserved. 综 述 1,2 1,2 1,2 1 1 1,2 1,2 方诩 ，秦玉琪 ，李雪芝 ，王禄山 ，汪天虹 ，朱明田 ，曲音波 1 250100 2 250100 : 利用纤维素酶将预处理后的秸秆降解成可发酵性单糖，然后发酵生产所需的液体燃料及化工产品的技术，对 于我国解决能源、环境、人口就业等难题有着巨大的积极影响。在木质纤维素生物降解转化工艺中，减少纤维素酶用 量及提高酶解效率是降低木质纤维素降解成本的关键。纤维素酶系和木质纤维素酶水解技术的改进需要深入了解纤维 素酶系统的组成及其协同作用、纤维素酶的结构与功能以及纤维素酶的生产技术。将就以上几个方面的研究进展进行 讨论，并深入探讨了纤维素酶糖化能力的评价方法。 : 纤维素酶，木质纤维素类生物质，纤维素酶解 Progress on cellulase and enzymatic hydrolysis of lignocellulosic biomass 1,2 1,2 1,2 1 1 1,2 Xu Fang , Yuqi Qin , Xuezhi Li , Lushan Wang , Tianhong Wang , Mingtian Zhu , and Yinbo Qu1,2 1 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China 2 National Glycoengineering Research Center, Shandong University, Jinan 250100, China Abstract: Biofuels and bio-based chemicals from lignocellulosic biomass are sustainable, making them alternatives to petroleum-derived fuels and chemicals to address the challenges of the shortage of crude oil supply and climate change resulted from the overconsumption of petroleum-based products, particularly in China. However, high cost in liberating sugars from lignocellulosic biomass is still the bottleneck of the commercialization of biofuels and bio-based chemicals. In this article, the major components of cellulases and their synergistic role in the hydrolysis of pre-treated biomass is reviewed, followed by how to evaluate the enzymatic hy