转基因稻米dna提取方法的比较研究-河南农业科学.pdfVIP

※分析检测 食品科学 2010, Vol. 31, No. 22 445 宋尚新，肖红梅，高 峰，周光宏 * ，邱良焱 (南京农业大学 教育部肉品加工与质量控制重点实验室，农业部农畜产品加工与质量控制重点开放实验室， 江苏 南京 210095) 目的：建立一种以水稻种子为原料，简便、快速、有效的基因组DNA 提取方法，作为转基因稻米的 PCR 检测基础。方法：选择传统的 CTAB 法、改良的碱处理法、高温水煮法，以转基因糙米、非转基因糙米、 转基因精米为材料提取 DNA ，并对提取物进行检测。结果：改良的碱处理法提取的DNA 模板的完整性、纯度和 PCR 反应方面与传统 CTAB 法相近，且比 CTAB 法的产率高、耗时短、成本低。结论：改良的碱处理法可以代 替传统的 CTAB 法用于转基因稻米检测中 DNA 模板的制备。 水稻；DNA 提取；改良的碱处理法；转基因检测 Comparison of DNA Extraction Methods for Transgenic Rice SONG Shang-xin，XIAO Hong-mei ，GAO Feng ，ZHOU Guang-hong* ，QIU Liang-yan (Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China) Abstract ：Objective: To establish a simple, fast and effective DNA extraction method from transgenic rice. Methods: DNA extraction methods including traditional CTAB method, improved alkali-treated method and boiling-treated method for DNA extraction from transgenic brown rice, non-transgenic brown rice and transgenic milled rice were compared to screen the optimal extraction method. Results: The integrity, purity and PCR result of template DNA extracted by improved alkali-treated method were similar with the DNA extracted by traditional CTAB method. Meanwhile, compared with traditional CTAB method, improved alkali-treated method had higher DNA extraction rate, less consuming time and lower cost. Conclusion: Improved alkali- treated method can replace traditional CTAB method as DNA extraction method for the detection of transgenic rice. Key words ：rice ；DNA extraction ；improved alkali-treated method ；detection of transgene 中图分类号：S511