374株中段尿培养阳性病原菌分布及耐药性分析.docVIP

精品论文 参考文献 374株中段尿培养阳性病原菌分布及耐药性分析 宝鸡市中心医院 陕西宝鸡 721008 【摘 要】目的：了解中段尿培养的病原菌分布及耐药情况,指导临床合理选用抗生素。方法：对374株病原菌用法国生物梅里埃公司VITEK 2-compact全自动细菌鉴定及药敏分析仪进行细菌鉴定和药敏试验。结果:1275例中段尿标本共分离出374株病原菌,其中革兰阴性菌260株,占69.52%；革兰阳性菌78株,占20.86%；真菌36株,占9.62%。最常见的革兰阴性菌为大肠埃希菌221株，占59.09%，几种常见的革兰阴性菌对亚胺培南均100%敏感；常见的革兰阳性菌对利奈唑烷﹑替加环素﹑万古霉素100%敏感。结论：中段尿培养阳性的主要病原菌为大肠埃希菌,此病原菌耐药率高易产生ESBLs酶，应规范合理使用抗生素，防止出现更高耐药性。 【关键词】中段尿培养； 病原菌； 耐药性；抗生素 【中图分类号】R969.4 【文献标识码】A 【文章编号】1674-8999（2015）9-0809-02 Distribution and drug resistance of pathogens in middle-segment urine 【Abstract】Objective：To investigate the distribution and drug resistance of pathogenic bacteria in the urine culture, guide the rational use of antibiotics in clinic. Methods：Of 374 strains pathogenic bacteria in biological merry Emmanuel France VITEK2-compact automatic bacteria identification and drug susceptibility analyzer to bacteria identification and drug sensitive test.Results 1275 cases of urine samples of 374 strains were isolated, including 260 strains of Gram-negative bacteria, accounting for 69.52%, 78 strains of Gram-positive bacteria, accounting for 20.86%, 36 strains of fungi, 9.62%. accounted for the most common gram negative bacteria was 221 strains of Escherichia coli, accounted for 59.09%, several common gram-negative bacteria are sensitive to the inferior amine and South；common gram-positive bacteria of linezolid, tigecycline, vancomycin.Conclusion Urine culture positive main pathogens were Escherichia coli, pathogen resistance rate high, rational use of antibiotics should be standardized, to prevent the emergence of more high drug resistance. 【Key words】middle-segment urine； pathogenic bacteria；drug ─resistance；drug ─fast antibiotics 1 引言 泌尿系统感染是由细菌引起的肾盂肾炎、膀胱炎、尿道炎等病的总称,是临床常见的感染性疾病。临床合理应用抗菌药物,对延缓细菌耐药性是至关重要的,而对病原菌的分布及耐药性的分析则是临床用药的重要依据。因此,了解中段尿培养的病原菌分布与耐药性分析,对临床合理选用抗菌药物有着重要的意义。 2 材料与方法 2.1标本来源 2012年5月至2013年5月我院住院、门诊送检的1275例中段尿培养标本。避免重复收集同一病人的同一菌株。 2.2试剂与仪器 采用法国生物梅里埃公司VITEK 2-compact 全自动细菌鉴