Origin作比生长速率步骤.doc

选中数据作直线图（以求比生长速率为例，第一栏数据是时间，第二栏数据是细胞浓度）； 在 ” Analysis”下拉框中选择”Non-linear Curve Fit”中的”Fitting Wizard”，然后一直选择Next，直至结束；此时在数据框中会出现模拟数据； 用模拟数据画直线图，然后选择” Analysis”中的”Calculus”中的”Differentiate”功能，可以求出生长速率； 此时再选择”Analysis”中的”Simple Math”，会出现一个对话框（如下所示），把细胞浓度那一栏”NLSF1_B”导入Y2中，operator 选择”/”，点击OK就可以求出比生长速率了。 其它的产物比合成速率以及底物消耗速率都依次类推就可以了。 Amplifying the manganese scavenging potential of Streptococcus zooepidemicus to reactive oxygen species during production of hyaluronic acid. more options Author(s): Mashitah, M D; Masitah, H; Ramachandran, K B Source: Med J Malaysia??Volume: 59 Suppl B????Pages: 59-60??Published: 2004 May ?? [ PubMed Related Articles ] Abstract: Streptococcus zooepidemicus (SZ) is an aerotolerant bacteria and its ability to survive under reactive oxidant challenge raises the question of the existence of a defense system. Thus growth, hyaluronic acid (HA) and hydrogen peroxide (H2O2) production by SZ in the presence of increasing concentration of Mn2+ were studied. The results suggested that the tested strain supported growth and HA production in cultures treated with 1 and 10 mM of Mn2+ regardless of H2O2 presence in the medium. This showed that SZ have acquired elaborate defense mechanisms to scavenge oxygen toxicity and thus protect cells from direct and indirect effect of this radical. In contrast, cells treated with 25 mM Mn2+ were sensitive, in which, the HA production was reduced considerably. Thus showing that the oxygen scavenger systems of the cells may be fully saturated at this concentration. PubMed ID: Document Type: Journal Article Language: English Address: Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. MeSH Terms: Heading Qualifier Dose-Response Relationship, Drug ? Free Radical Scavengers *metabolism Humans ? Hyaluronic Acid *biosynthesis Hydrogen Peroxide *metabolism Magnesium *pharmacology Reactive Oxygen Species *metabolism Streptococcus equi *drug