Resveratrol对人外周血T细胞活化的影响及机制研究.docVIP

精品论文 参考文献 Resveratrol对人外周血T细胞活化的影响及机制研究 夏菲 邹婷 张进平(通讯作者) 　　（苏州大学生物医学研究院 215123） 　　【摘要】目的 本研究旨在探讨Resveratrol对人T 淋巴细胞活化的影响及其分子机制。方法　在体外使用Resveratrol处理人外周血T 细胞，并给予抗人CD3和抗CD28抗体活化，观察其对T细胞增殖、细胞因子分泌及细胞凋亡的影响。同时进一步通过免疫印迹和免疫共沉淀的方法探讨Resveratrol对T细胞活化影响的分子机制。结果　我们现有的实验提示Resveratrol可以明显抑制人外周血T 淋巴细胞的增殖，且能抑制IL-2、IL-4、IL-5、IFNgamma;等细胞因子的分泌；但并不影响T细胞的凋亡。进一步研究表明Resveratrol通过提高Sirt1的表达和增强其对C-jun的脱乙酰化来发挥作用。结论　此研究提示resveratrol可能在T细胞引起的炎症如自身免疫性疾病治疗中发挥重要作用。 　　【关键词】 Resveratrol T 细胞活化 脱乙酰化 C-jun Sirt1 　　【中图分类号】R392 【文献标识码】A 【文章编号】2095-1752（2014）02-0152-03 　　The Roles and Molecular Mechanisms of Resveratrol on Human T cell Activation 　　XIA Fei, ZOU Ting, ZHANG Jin-ping 　　(Institutes of Biology and Medical Sciences, Soochow University, Suzhou, 215123 Jiangsu Province, China) 　　【Abstract】 Object: We aim to explore the function and molecular mechanisms of resveratrol on human T cell activation. Method: The PBMC were obtained from health people, and purified by Ficoll-hypaque, the T cell were activated by anti-CD3 and anti-CD28 antibodies, the proliferation was evaluated by CFSE cell division assay and Brdu cell proliferation assay. The levels of cytokines in activated T cells culture supernatant were measured by ELISA. The expression of Sirt1 and alteration of acetylation of C-Jun were evaluated by western-blot. Result:We found that Resveratrol significantly inhibited the activation and cytokines production of T cell in hunman PBMC. The expression of Sirt1 was up-regulated and acetylation of C-Jun was decreased in resveratrol-treated T cells. Conclusion: Resveratrol inhibited the T cell activation by up-regulating the expression of Sirt1 and enhancing the deacetylase activity of Sirt1 on C-Jun in T cells. 　　【Key Words】 Resveratrol Sirt1 T cell activation deacetylase C-Jun 　　Resveratrol是一种天然的多酚化合物，富含在桑椹、花生、葡萄等植物中[1, 2]。自从人们发现饮用红葡萄酒的人能抵抗冠心病以来，Resveratrol得到了科学家的广泛关注[3]。很多文献报道Resveratrol在炎症、氧化、癌症以及衰老中发挥重要作用[4-8]。Subhas