TGFβ基因调控对肾组织超微结构与肾功能影响的研究.docVIP

精品论文 参考文献 TGFβ基因调控对肾组织超微结构与肾功能影响的研究 谢宏发 王小荣 刘鲜桂 　　（广东省东莞三局医院儿科 523710） 　　【摘要】目的 探讨转化生长因子beta;(TGFbeta;)在肾病综合征患儿血、尿及肾组织中的表达及其在发病中的作用。方法　采用常规病理、免疫组织化学方法对肾组织进行染色，并经病理图像分析系统进行分析；血、尿TGFbeta;水平采用酶联免疫吸附法测定。结果　肾病组患儿的血、尿中TGFbeta;的含量明显高于正常对照组(Plt;0.05)；TGFbeta;在正常对照组的肾小球无表达，而在肾病组患儿肾小球及肾小管中有较密集的棕黄色颗粒，图像分析系统计算显示肾病组肾组织中的TGFbeta;表达较正常对照组显著增多，差异具有统计学意义(Plt;0.05)。结论　肾病综合征患儿血、尿及肾组织中TGFbeta;表达显著增加，可能在肾脏纤维化过程中发挥重要作用。 　　【关键词】 TGFbeta; 基因调控 肾组织超微结构 肾功能 　　【中图分类号】R692 【文献标识码】A 【文章编号】2095-1752（2014）06-0093-02 　　 　　TGF beta gene regulation effects on renal function and renal tissue ultrastructure of research 　　【Abstract】 objective: to investigate the transforming growth factor beta (TGF beta) in children with nephrotic syndrome in the blood, urine and kidney tissues expression and its role in the pathogenesis. Methods: routine pathologic and immunohistochemical method to dyeing of kidney tissues, and pathological image analysis system were analyzed; Blood and urine TGF beta level by using enzyme-linked immunosorbent determination. Results: the blood of children with kidney disease group, urine content of TGF beta was obviously higher than that of normal control group (P lt; 0.05); Glomerular no expression of TGF beta in the normal control group, and in children with nephrotic group of glomerulus and renal tubule has more intensive tan grain, image analysis system calculation shows nephropathy group compared with normal kidney tissues of TGF beta expression was significantly increased, statistically significant difference (P lt; 0.05). Conclusion: children with nephrotic syndrome in the blood, urine and kidney tissues TGF beta expression significantly increased, may play an important role in the process of renal fibrosis. 　　【Key words】 TGF beta Gene regulation Kidney tissue ultrastructure Renal function 　　儿童原发性肾病综合征(PNS)是儿科常见的肾小球疾病之一，血IgG水平降低、细胞功能紊乱等提示本病的发生与机体免疫异常有关。既往资料也提示肾小球基底膜通透性增加是致大量蛋白从尿中漏出的重要原因[1]。随机选择我院2011年9月-2013年