乙肝HBeAg 定量、前S1 蛋白及HBV-DNA之间的相关性探讨.docVIP

精品论文 参考文献 乙肝HBeAg 定量、前S1 蛋白及HBV-DNA之间的相关性探讨 青岛大学医学院免疫学教研室 山东青岛 266071 【摘 要】目的 探讨乙肝患者HBeAg 定量、前S1 蛋白及HBV-DNA 之间的相关性。方法 乙肝HBeAg 定量采用电化学发光免疫法，同时采用荧光定量PCR 法检测HBV-DNA，酶联免疫法检测前S1 蛋白。结果 HBeAg 阳性与HBeAg 阴性模式中HBV-DNA 阳性率分别为83.5%和45.5%，差异有统计学意义（chi;2 =23.7，Plt;0.01），前S1 蛋白的阳性率分别为75.0%和36.0%，差异有统计学意义（chi;2 =19.9，Plt;0.01）。HBeAg 和前S1 蛋白检测模式中，双阴性组，单阳性组和双阳性组之间HBV-DNA 阳性率差异均有统计学意义（Plt;0.01）。HBeAg 和前S1 蛋白联合检测与 HBV-DNA 之间的符合率为78.5%。HBeAg 与HBV-DNA双阳性的样本中，两指标之间成正相关（r=0.392，Plt;0.01），对HBV-DNA 四组间进行两两比较，发现lt;103组和103-5组HBeAg数值无明显差异（Pgt;0.05），其余各组间差异均有统计学意义（Plt;0.05）。结论 HBeAg、HBV-DNA、前S1 蛋白三者有较好的相关性，HBeAg 和前S1 蛋白联合检测与 HBV-DNA 之间符合率较好，且HBeAg 含量与HBV-DNA 含量之间成正相关。 【关键词】乙肝；HBeAg；HBV-DNA；前S1 蛋白 Comparative analysis on detection results of HBV HBeAg，preS1-Ag and HBV-DNA[Abstract] Objective To study the correlation between HBeAg and Pre-S1 and HBV-DNA antigen in hepatitis B patients.MethodsHepatitis B HBeAg adopt ECLI，while using real-time PCR to detect HBV-DNA，enzyme-linked immunosorbent assay pre-S1protein.Results The positive rate of HBV-DNA of HBeAg positive and negative group is 83.5% and 45.5%，the difference was statisticallysignifican（t chi;2 = 23.7，P lt;0.01），the positive rate of Pre-S1 protein is respectively75.0% and 36.0%，the difference was statistically significant（chi;2 = 19.9，P lt;0.01）.HBeAg and pre-S1 protein combined detection，HBV-DNA positive rate differences were statistically significant（P lt;0.01）between the double-negative group，positive group of single and double-positive group.The compliance rate between HBeAgand pre-S1 protein combined detection and HBV-DNA is 78.5%.For HBeAg with HBV-DNA double positive samples，there is a positivecorrelation between the two indicators（r = 0.392，P lt;0.01），in the four groups of HBV-DNA，there is no significant difference of HBeAglevel between lt;103 group and 103-5 group（Pgt; 0.05），among other groups differences were statistically significant（P lt;0.05）.ConclusionHBeAg，HBV-DNA，Pre-S1 antigen has a good correlation