水稻白叶枯病菌酸性还原酮加双氧酶的鉴定分析.pdfVIP

植物病理学报 ACTAPHYTOPATHOLOGICASINICA 44(1)：46—53(2014) 水稻白叶枯病菌酸性还原酮加双氧酶的鉴定分析 夏更寿h，张燕2，宋从凤2，王金生2 (1丽水学院生态学院，丽水323000；2南京农业大学植物保护学院分子植物病理学国家重点实验室，南京210095) 摘要：酸性还原酮加双氧酶(ARD)催化很多原核和真核生物中的甲硫氨酸急救途径(MSP)倒数第二步。本研究鉴定了水 稻白叶枯病菌Xanthomonasoryzae 该基因突变对Xoo在水稻上的毒性没有影响。 关键词：Xanthomonas oryzaepv．oryzae；酸性还原酮加双氧酶；鉴定；分析 Identification and ofacireductone fromXanthomonas analysis dioxygenase oryzae XIA of pv．oryzaeGeng．shoul，ZHANGYan2，SONGCong：fen92，WANGJin—shen92(1College State of MolecularPlant ofPlantProtec— Ecology，LishuiUniversity，Lishui323000，China；2KeyLaboratory Pathology，College Agricultural 210095，China) tion，Nanjing University，Nanjing Abstract：Acireductone an the ofthemethionine dioxygenase(ARD)isenzymecatalyzingpenultimatestep and the inXan- salvagepathway(MSP)inubiquitousprokaryoticeukaryoticorganisms．Herecounterpartgene thomonas xardwasidentified．The oryzae nucleotide ofxardsharedthe pv．oryzae(Xoo)，named sequences entireidentitiesinXoostrains 1018andKACCl0331．Thestrainwithaninactiveformof PX099A，MAFF31 xardcouldnot when theexclusivesulfursource．Thisresult grownormallymethylthioadenosine(MTA)as that confirmedXardfunctionedinMSP．Postinoculationofxardmutantandwild strainPX099Aonrice type line of thatmutationof lesion data15 showed xard hadnoeffectonvirulenceof IR24，thelength day