虫草花多酚浸提工艺优化及抗氧化活性研究.pdfVIP

Advances in Analytical Chemistry 分析化学进展, 2017, 7(4), 240-248 Published Online November 2017 in Hans. /journal/aac /10.12677/aac.2017.74032 Optimization of Extraction Technology of Total Polyphenols in Corhyceps Flowers and the Antioxidant Effects * Wubiao Zhu , Yong Hou, Xiaomei Zhuang Department of Applied Chemistry, Zhongshan Polytechnic, Zhongshan Guangdong th nd th Received: Nov. 10 , 2017; accepted: Nov. 22 , 2017; published: Nov. 29 , 2017 Abstract In this study, the extraction technology of total polyphenols from Corhyceps flowers was optimized through single factor experiment and orthogonal experiment. Then, the extraction was washed by petroleum ether, ethyl acetate and n-butanol. The effects of total polyphenols from different part in Corhyceps flowers on the scavenging of ABTS+ radicals, DPPH radicals and alpha-glucoside in- hibition were also studied. The result shows that the factors effected the Gallic acid content are the ethanol extraction extraction temperature solid-liquid ratio extraction time and the best craft is using solid-liquid ratio 1:25, the content of ethanol is 80%, extraction temperature is 80˚C, the extraction time is 1 h. The ethyl acetate fraction of Corhyceps flowers, a strong ability to act as antioxidant, might be considered as a natural source of active compounds. Keywords Cordyceps, Total Polyphenols, Extraction 虫草花多酚浸提工艺优化及抗氧化活性研究 * 朱屋彪 ，侯 勇，庄晓梅 中山职业技术学院应用化学教研室，广东 中山 收稿日期：2017年11月10 日；录用日期：2017年11月22 日；发布日期：2017年11月29 日 摘 要 本文研究虫草花多酚浸提最优工艺，通过单因素试验和正交试验结合，以总多酚含量为优化指标。然后 *通讯作者。 文章引用: 朱屋彪, 侯勇, 庄晓梅. 虫草花多酚浸提工艺优化及抗氧化活性研究[J]. 分析化学进展, 2017, 7(4): 240-248. DOI: 10.12