比较两种HBVDNA荧光定量PCR检测试剂.pdf

论著ONTHE 比较两~~HBVDNA荧光定量PCR检测试剂 刘靳波 徐 漂 (1．泸州医学院附属医院检验科 2．泸州医学院医学检验系 646000) 【摘 要l目的 比较一种新型乙型肝炎病毒 (HepatitisBvirusQ，HBV)核酸荧光定量PCR检测试剂 (以下简称 “新 型定量试剂”)与凯杰HBVDNA定量试剂的灵敏度、定量范围和临床应用价值。方法 以圣湘生物技术有限公司的新型HBVDNA PCR定量检测试剂 (磁珠法)为实验试剂，选取 36例 已知定量结果的血清标本为研究对 象，采用新型定量试剂进行定量检测， 运用SPSS17．0统计学软件进行配对 t检验及相关性分析，对新型定量试剂的灵敏度、定量范围等进行评价。结果 与凯杰HBV DNA定量试剂 (煮沸法)检测结果比较 ，经新型定量试剂检测的 36例标本中，两者结果均大于5O0IU／ml的标本 13例，阳性率 一 致性为 100％，阴性率一致性86．9％。将两次结果均为阳性的标本取对数值后，经统计分析 ，两检测结果具有较好的相关性， 结果无显著差异。新型定量试剂的最低检测限为20IU／ml，定量范围为2O一2x109IU／ml，凯杰试剂最低检测限为500Iu／ ml，定量范围为5OO一5xl08 IU／ml，新型定量试剂的灵敏度更高，定量范围更宽。结论 新型定量试剂的操作简便、省时省力、 灵敏度高、定量范围宽等优点，具有较好的临床应用价值。 l关键词】乙型肝炎病毒 荧光定量PCR 磁珠法 Com parison oftwoHBV DNA fluorescencequantitativePCR reagents Jinbo Liu， Piao Xu Department of laboratory medicine， Affi1iated Hospital of Luzhou Medical College 【Abstract] Objective Comparison ofa new hepatitisB virus (HepatitisB virus Q，HBV)nucleicacid fluorescence quantitative PCR reagents (hereinafter referred toas ”new quantitative reagent”) and QIAGEN HBV DNA quantification reagent sensitivity． quantitative range and c1inical application．Methods Xiang Biotechnology Co．．Ltd．in st．nove1HBV DNA PCR quantitative detection reagents (beads) as experimental reagents， selected 36 cases known quantitative resuits of serum samples for the study， the use of new quantitative reagent for quantitative detection。 using SPSS17．0 paired t—test and correlation ana1Ysis staristical software．Result 36 cases specimens were new quantitative reagent test results compare with the original data，the resultswere more than 5001U／mlspecimensin 13 cases，the positive ratewas 100％ consistency． consistency negative rate was 86．9％． The two samples were positire after taking the value， the statistical analysis， the two test results with good correlation， showed no significant differences．The new qu