运动对高胆固醇饮食小鼠主动脉脂质沉积影响的透射-天津体育学院学报.PDFVIP

2002 年 第 17 卷 第 3 期 　　　　　　　　　　　　　　　　　　　J our n al of Tj I P E Vol . 17 No . 3 ,2002 8～10 天津体育学院学报 　 ●　成果报告 　Original Article s 　 运动对高胆固醇饮食小鼠主动脉脂质沉积影响的透射电 镜观察 1 2 3 李 　瑞 ,刘善云 ,陈家琦 ( ) 摘要 : 目的:建立高胆固醇饮食诱导动脉粥样硬化 AS 小鼠模型 ,透射电镜观察有氧运动对高胆固醇饮食小鼠主动脉壁的影响。方法 : C57BL/ 6J ( ) ( ) ( ) C57B6 小鼠 19 只 ,随机分为高胆固醇饮食对照组 n = 10 和高胆固醇饮食运动组 n = 9 ,实验为期 12 周 。取主动脉以醋酸铀及柠檬酸盐染色 ,进 行透射电镜观察 。结果 :高胆固醇饮食对照组小鼠主动脉内皮及中膜平滑肌损伤严重 ,而运动组病变轻微 。结论 :运动可减少或防止高胆固醇饮食小 鼠主动脉高血凝状态 ,并能明显地减少胆固醇的沉着及其相关病损 。 关键词 : 动脉粥样硬化 ;高胆固醇饮食 ;有氧运动 ;主动脉壁 中图分类号 : G804 . 8 　文献标识码 : A 　文章编号 : (2002) Observation of Transmission Electron Microscope On Eff ect of Exercise on Aortic L ipid Sediment of Mice Fed with Hypercholesterol Diet L I Rui1 , L IU Shanyun2 , CHEN J iaqi3 ( 1. Dept . of Physical Education , Tianjin Institute of Physical Education , Tianjin 300381 ,China ; 2 . Dept . of Social Sport and Manage ment , Tianjin Institute of Physical Education , Tianjin 300381 , China ; 3 . Research Institute of Sport s Medicine , Tianjin Institute of Phys ical Education . Tianjin 300381 , China . ) Abstract : Aims : The at herosclerosis (AS) model of mice was set up induced wit h hypercholesterol diet . Transmission elect ronmicroscopic was used to ob serve t he effect of aerobic exercise on t he mice aorta wit h hypercholesterol diet . Met hods :Nineteen C57B6 mice were divided into cont rol group ( n = 10) wit h hypercholesterol diet and t he group of hypercholesterol diet plus exercise ( n = 9) . After 12 weeks of t he experiment , t he aortas were stained wit h uranium acetate and cit rate , observed wit h elect ronmicroscopic . Result s : The endot helium and smoot h muscle of aorta of group of hyper