早期吸入糖皮质激素对哮喘大鼠气道重建及NF—κB和IGF—1表达的影响研究.pdfVIP

摘 要 目的:研究早期吸入糖皮质激素对气道重建及核因子一KB (NF-YB),胰 岛素样生长因子一1(IGF-1)表达的影响。方法:将30只大鼠随机分为对照组、 哮喘组和地塞米松治疗组。免疫组织化学技术检测气道壁NF-xB,IGF-1的表 达，计算机图像分析技术对气道壁NF-xB和IGF-1的表达进行定量分析，及测 量小气道内周长和厚度，并观察嗜酸性粒细胞浸润情况。结果:NF一xB及IGF-1 的表达水平、嗜酸性粒细胞数、气道壁厚度哮喘组显著高于对照组((P0.05); 治疗组显著低于哮喘组((P0.05);但与对照组相比仍较高((P0.05);IGF-1的表 达水平与气道壁厚度正相关。结论:哮喘时气道壁增厚，气道重建，NF-xB和 IGF-1的表达增加，早期糖皮质激素治疗可减轻气道壁厚度增加，减少NF-xB 和IGF-I的表达。 关键词:哮喘;气道重建;核因子一KB;胰岛素样生长因子一1;糖皮质激 素 Effectsofearlyinhaledcorticosteroidsonairwayremodeling,expressionof nuclearfactor-kappaBandinsulin-likegrowthfactor-Iinasthmarats. Abstract 0句ective:Toinvestigatetheeffectsofearlyinhaledcorticosteroidsonairway remodeling,theexpressionofnuclearfactor-kappaB(NF-B‘)andinsulin-likegrowth factor-I(IGF-1)inasthmarats.Methods:Thirtyratswererandomlydividedinto threegroups:controlgroup,asthmagroupandtherapeuticgroupwithinhaled dexamethasone.TheexpressionofNF-xBandIGF-1inlungtissuewereobservedby immunohistochemicalstaining.Withcomputerimageanalysissystem，wedetected theairwayexpressionofNF-xBandIGF-1，thethicknessofairwaywall，andthe numberofeosinophils.Results:TheexpressionofNF-xBandIGF-1，the thicknessofairwaywallandthenumberofeosinophilsweresignificantlyhigherin asthmagroupthanthoseofcontrolgroup;Therewerestatisticaldifferencebetween thetherapeuticgroupandthecontrolgroup，buttheexpressionofNF-xBandIGF-1， thethicknessofairwaywallandthenumberofeosinophilsweresignificantly decreasedintheairwayoftherapeuticgroupcomparedwithasthmaticgroup. Conclusion:Thethicknessofairwaywallincreasesinasthma,theairwayremodles andtheexpressionofNF-xBandIGF-1rises.Thetreatmentofearlyinhaled corticosteroidscaninhibitincreaseofairwaywallthicknessanddecreasethe expressionofNF-xBandIGF-1， KeyWords:asthma;airwayremodeling;nuclearfactor-kappaB;insulin-like growthfactor-I;corticosteroid 文 献 综 述 传统观点认为哮喘的特征是气道慢性炎症，可逆性的气流阻塞。但近年大量 研究发现哮喘