八角莲组织培养的器官发生途径研究-广西植物.pdf

DOI:10.11931/guihaia.gxzw201612025 八角莲组织培养的器官发生途径研究 1 1 1 1 1 1* 陈 瑶 ，刁 瑕 ，宦云敏 ，杜阳春 ，李 维 ，何 兵 (1.四川师范大学 生命科学学院，四川 成都 610101） 摘要：为探究小檗科植物八角莲组织培养的器官发生方式，以八角莲离体叶片、叶柄在MS培养基上诱导 产生的愈伤组织、不定芽、不定根为研究对象，用连续石蜡切片技术分析八角莲组织培养的器官发生途径。 结果表明：八角莲愈伤组织形成的解剖学特征是靠近表皮的薄壁细胞经激素刺激恢复分裂能力，继续培养 形成拟分生组织。拟分生组织可形成许多分化中心。通过对八角莲组织培养产生的不定芽细胞组织学观察 发现芽原基起源于愈伤组织外侧的几层薄壁细胞，芽原基背离愈伤组织中央生长形成不定芽，故八角莲脱 分化形成的芽起源方式为外起源。而八角莲的根原基起源于组织深处髓部薄壁细胞和部分维管形成层细 胞，进而形成类似球形或楔形并朝韧皮部突起的根原基轮廓，根原基继续发育会突破表皮生成不定根，起 源方式为内起源。八角莲离体再生常规途径为器官发生型，在组培苗生长过程中先诱导形成不定芽，再诱 导形成不定根，在愈伤组织上形成维管组织将不定芽和不定根连接成完整植株。 关键词：八角莲；不定芽；不定根；组培苗；石蜡切片 中图分类号： 文献标志码：A 论文编号： Organogenesisapproachesintissueculture ofDysosma versipellis 1 1 1 1 1* CHENYao ,DIAOXia ,HUANYun-Min,DUYang-Chun ,LIWei ,HEBing （1.Collegeof LifeScience,SichuanNormal University,sichuan Chengdu 610101,China） Abstract:To study the organic regeneration pathway of tissue culture of Dysosma versipellis, based on the technique of serial paraffin slices, we used callus, adventitious shoots and roots induced from in vitro leaves and petioles of Dysosma versipellis on MS medium to analyze it. The results showed that the parenchyma cell of it near the epidermis recovered cell division ability through the hormone stimulation. Originally cells of the stationary state altered cell metabolism after were stimulated, cell division become active, this organization is by the meristem and alienation a variety of cell types,then continued to culture callus formed meristemoid with cells division potential of totipotency of plant cell, which can form a lots of differentiation center. Histological observation of Dysosma versipellis proved the bud primodium derived from the