TDZ对蓝莓茎段和叶片离体培养的影响.docx

天津农学院毕 业 论 文 中文题目:TDZ对蓝莓茎段和叶片离体培养的影响 英文题目:Effects of TDZ on Blueberry Stem Section andLeaf Culture in Vitro 学生姓名 杨坤 二级学院 园艺园林学院系 别 园艺系 专业班级 2013 级园艺专业蔬菜班 指导教师 柴慈江 成绩评定 2017年6月目 录1 前言12 材料与方法22.1 试验材料22.2 试验方法23 结果与分析33.1 不同TDZ浓度对同一蓝莓品种下茎段的影响33.2不同TDZ浓度对同一蓝莓品种下叶片离体的影响43.3 TDZ浓度和品种处理组合对蓝莓试管苗茎段培养的影响53.4不同品种下相同TDZ浓度对蓝莓茎段的影响83.5 不同品种下相同TDZ浓度对蓝莓叶片离体的影响93.6 品种与TDZ浓度处理组合对蓝莓叶片离体培养的影响94 讨论114.1 不同TDZ浓度对不同品种蓝莓茎段的影响 114.2 不同TDZ浓度对不同品种蓝莓离体叶片的影响114.3 不同蓝莓品种对TDZ培养效果的差异性125 结论13参考文献14致谢15附录1 相关英文文献16附录2 英文文献中文译文21摘 要此项研究有关TDZ对于蓝莓茎段和叶片离体培养的影响。在附加6-BA0.5mg/L和0.1mg/L的IBA的WPM培养基中，分别加入0、0.003、0.006、0.012、0.018mg/L的TDZ，接种蓝莓试管苗茎段或叶片进行培养。结果表明当TDZ浓度为0.003 mg/L时北陆蓝莓茎段培养结果的各项指标达到最佳，繁殖系数为16.5；当TDZ浓度为0.012mg/L时，蓝丰和北陆蓝莓叶片离体培养结果的各项指标达到最佳，且叶片的芽分化率为53.3%，每叶片平均分化1.37个芽。TDZ可促进北陆茎段培养中繁殖系数的提高，但对斯巴坦和瑞卡的茎段培养有抑制作用。在含有不同浓度TDZ的培养基中，北陆、蓝丰和爱国者3个蓝莓品种的叶片离体培养效果没有表现出明显的差异。关键词: 蓝莓；试管苗；TDZ；茎段培养；叶片培养ABSTRACTThe aim of this study was to investigate the effects of TDZ on the in vitro culture of stem segments and leaves of blueberry. With 0, 0.003, 0.006, 0.012, 0.018mg/L TDZ added in WPM medium with 6-BA0.5mg/L and 0.1mg/L IBA, the stem section culture or leaf culture of blueberry were carried out. The results showed that when TDZ was 0.003mg/L the propagation coefficient of the blueberry stem sections was 16.5 which was the best. With the TDZ concentration of 0.012mg/L the leaf culture had the best effect, with bud differentiate rate of 53.3% and bud number of 1.37 per leaf.TDZ could increase the propagation coefficient of the “north land’ blueberry stem sections but could decline the propagation coefficient of the “Sbutan” and “Ruika” blueberry stem sections. In media with different concentration of TDZ the leaf culture effects had no significant difference among “Patriot ”, “Blue crop” and “north land” blueberry cultivars.Keywords: Blueberry; plantlet in vitro;TDZ;stem section culture; l