海蜇糖蛋白对小鼠脾脏细胞因子mRNA表达的影响-河南农业科学.PDFVIP

※营养卫生 食品科学 2013, Vol.34, No.23 305 海蜇糖蛋白对小鼠脾脏细胞因子mRNA 表达的影响 任国艳，邵 征，曹 力，曹 利，张 凡，郭金英，樊金玲，陈秀金，赵瑞红 (河南科技大学食品与生物工程学院，河南 洛阳 47 1003) 摘  要：研究海蜇糖蛋白(JGP-III)对BALB/c小鼠脾脏淋巴细胞细胞因子mRNA表达的影响，探讨JGP-Ⅲ对小鼠的免 疫调节机理。选用54 只BALB/c小鼠(18～22g ，SPF级) ，随机分为6组：模型对照组、正常对照组、阳性对照组(左 旋咪唑25mg/(kg ·d) ，以体质量计) 、低剂量组(JGP-Ⅲ 10mg/(kg ·d)) 、中剂量组(JGP-Ⅲ 50mg/(kg ·d))和高剂量组 (JGP-Ⅲ 100mg/(kg ·d)) ，每组9只。饲养2 1d后，无菌取脾脏，分离淋巴细胞，采用荧光定量RT-PCR法分析其细胞 因子IL-2 、IL-12 、IFN-γ 、IL-4 、IL-6和IL-10 mRNA 的相对表达量。结果显示：通过3个剂量组和3个对照组中细胞 因子mRNA表达水平的对比，得出海蜇糖蛋白JGP-III能够促进细胞因子IL-2 、IL- 12 、IFN-γ 的mRNA表达水平，而 抑制IL-4 、IL-6和IL-10 mRNA 的表达水平，且具有剂量依赖关系。进而推测JGP-Ⅲ可能是通过调节淋巴细胞Th 1/Th2 细胞因子mRNA 的表达水平，来提高机体免疫能力。 关键词：海蜇；糖蛋白；细胞因子；mRNA表达 Effects of A Glycoprotein from Jellyfish (Rhopilema esculentum) on Cytokine mRNA Expression in BALB/c Mice REN Guo-yan，SHAO Zheng，CAO Li，CAO Li，ZHANG Fan，GUO Jin-ying，FAN Jin-ling，CHEN Xiu-jin， ZHAO Rui-hong (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 47 1003, China) Abstract ：In a previous study, we isolated a glycoprotein named JGP-III from j ellyfish (Rhopilema esculentum). The spe- cific aim of the present study was to investigate the roles of JGP-III in the immunoregulatory mechanism in BALB/c mice by investigating its effects on the mRNA expression of the Th1- and Th2-cytokines in the spleen lymphocytes of these mice. A total of 54 healthy BALB/c mice (SPF, 18－22 g) were randomly divided into 6 groups with 9 mice in each group: normal control, model control, positive control, low dose (10 mg/(kg ·d)), moderate dose (50 mg/(kg ·d)) and high dose (100 mg/(kg ·d)) groups, re- spectively. After 21 days of these treatments, lymphocytes were prepared from the spleen of these mice