地塞米松对溃疡性结肠炎鼠外周血及肠黏膜固有层淋巴细胞凋亡的影响.pdfVIP

·386· 匹耍匡药2Q13生5旦箜堡鲞蔓5塑』i旦望匝丛!垂i趔』!坠里尘，丛型2Q!兰，y!!堡，盟煎 ．实验研究· 地塞米松对溃疡性结肠炎鼠外周血及肠黏膜固有层淋巴细 胞凋亡的影响 李志晋，詹丽英，胡俊，郑闽，徐燕琴，马春曦 (江西解放军第184医院消化科，鹰潭335000) 摘要：目的观察溃疡性结肠炎(UC)大鼠外周血淋巴细胞(PBL)及肠黏膜固有层淋巴细胞(LPL)凋亡状态，并探讨地塞米 松(Dex)对其的影响。方法将45只大鼠随机分为正常组、模型组、实验组3组，各15只，模型组及实验组用三硝基苯磺酸 (rI’NBSl，乙醇灌肠制作实验性溃疡性结肠炎的动物模型，正常组以生理盐水灌肠作为对照，分离各组大鼠PBL及LPL，植物血 少．而PBL无明显凋亡异常，LPL凋亡减少可能同溃疡性结肠炎发病机制有关，地塞米松通过促进Ⅱ)L凋亡从而达到治疗作 用。 关键词：溃疡性结肠炎；凋亡；肠黏膜固有层淋巴细胞；外周血淋巴细胞；地塞米松 中图分类号：R一332文献标识码：B 文章编号：1006—2238(2013)05—0386—03 DOI：10．3969，j．issn．1006—2238．2013．05．005 EffectsofDexamethasonon of blood andlaIIIina inulcerativecol- apoptosisperipherallymphocytesproprialymphocytes irisratsLI ZhiJin,ZHANJun，et ofGastroenterology，184ofPLA，Yingtan335000,China Liying,HU01．Department Hospital Toobservethe in blood lamina proprialymphocytes Abstract：Objectiveapoptosisperipherallymphoeytes(PBLs)and ulcerativecolitisratsand effectsof them．Methods catswere (LPIJs)from investigate Dexamethason(Dex)onForty—fivehealthy modelof wasestablished dividedintothree normal model rat UC randomly groups：the group，the group，theexperimentalgroup．111e alcoh01．PBLsandLPLswereisolatedfromeach weredetect- byusingTrinitrophenylmethylnitramine(TNBS)and group．Apoptosis ed flow afterstimuilated for72hours．PBLsandLPkfrom werecultured by eytometry byphytohemagglutinin experimentalgroup with the indexs after The ofLPLsfromthemodel culturedinvitrowas Dexand same were culture．Results group go