向日葵菌核病生防放线菌的分离筛选及拮抗作用的初探-华北农学报.PDFVIP

华北农学报·2006, 21(1):96-99 向日葵菌核病生防放线菌的分离 筛选及拮抗作用的初探 1 2 1 1 1 胡　俊 ,刘正坪,周洪友 ,丁　俊,宋　娟 (1.内蒙古农业大学 农学院, 内蒙古呼和浩特　010019;2.北京农学院植物科学技术系, 北京　102206) 　　摘要:根据微生物间的拮抗作用, 试从病地土壤中分离拮抗放线菌, 探索利用放线菌对向日葵菌核病进行生物防 治的途径。从内蒙古呼和浩特市、巴彦淖尔、包头等地所采集的土壤中分离出38 个放线菌菌株, 经过平板拮抗作用初 测, 有8 个菌株(F-1、F-3、F-6、F-8、F-11、F-15、F-21、F-25)对向日葵核盘菌(Sclerotinia sclerotior um )有拮抗作 用。选出其中2 个作用最明显的菌株(F-6、F-8), 观察其在平板对峙培养中对向日葵核盘菌的抑制作用及其代谢产 物对向日葵核盘菌的作用。结果表明:F-6、F-8 菌株及其代谢产物对向日葵核盘菌均有明显的抑菌作用。 同时, 对F-6、F-8 两菌株的培养性状和形态特征进行了初步观察。 关键词:向日葵;核盘菌;拮抗放线菌;代谢产物 中图分类号:S435.655　　文献标识码:A　　文章编号:1000-7091(2006)01-0096-04 Studies on Isolation and Screening of Antagonisticmycetes-fungus and Its Antagonistic Effect for Sunflower Sclerotinia Rot 1 2 1 1 1 HU Jun ,LIU Zheng-ping ,ZHOU Hong-you , DIN Jun ,SON Juan (1.Inner MongolicAgricultural University,Huhhot　010019,China; 2.Beijing Agricultural College,Beijing　102206,China) Abstract:We explored the way of biological control by isolating antagonisticmycetes-fungus from pathogenic soil for Sclerotinia sclerotiorum .The result showed that 38 strains were isolated from soil samples derived from Huhhot,Bayannao- er and Baotou regions of Inner Mongolia.We observed that 8 strains,named as F-1,F-3,F-6,F-8,F-11,F-15, F-21,F-25, displayed antagonisms against S .sclerotiorum on the PDA plate.F-6 and F-8 were selected to study their inhibition to S .sclerotiorum growth and effects of their metabolic products on S .sclerotiorum of sunflower.Meanwhile,we examined the cultural and morphological characteristics of F-6 and F-8.Results showed that F-6,F-8 and their metabolic products could inhibit S .sclerotiorum growth