阳离子脂质体介导的基因转移机理及其应用.pdfVIP

阳离子脂质体介导的基因转移机理及其应用 * 王冰 张树彪 杨宝灵 崔绍辉 赵轶男 （大连民族学院，辽宁大连，116600） 摘要：基因治疗在有效进行遗传性和获得性疾病治疗方面，取得重要进展。与病毒载体相比, 许多天然的和合成的阳离子类脂以脂质体的形式用于基因转移，具有无免疫原性、易生产、 质粒免受核酸酶降解、无致瘤性等优点。作为病毒载体的有效替代，阳离子类脂被用于细胞 的体内和体外转染。类脂构效关系的研究和基因转移机理的揭示是提高转染效率和优化基因 治疗的关键。本文综述了阳离子脂质体介导的基因转移机理：阳离子脂质体/DNA 复合物的 形成，细胞结合吸收载体，内含体释放和复合物解体，细胞核摄入。通过阳离子脂质体介导 的pGFP-N2 转染Hela 和MCF-7 细胞研究，验证了脂质体运载pDNA 的有效性。MTT 法也得到 了理想结果。 关键词：脂质体；基因转移；机理；Hela ；MCF-7 中图分类号：Q783 Mechanisms involved in cationic lipid mediated gene transfer and its application Bing Wang ,Shubiao Zhang*, Baoling Yang ,Shaohui Cui ,Yinan Zhao (Colledge of Life Science, Dalian Nationalities University, Dalian Development Zone 116600) Abstract: Gene therapy represents an important advance in the treatment of both genetic and acquired diseases. Many cationic lipids naturally occurred or synthesized have been used for gene transfer in the form of liposomes, for they have the advantages over viral gene transfer as non-immunogenic, easy to produce, resistant to nuclease degradation and not oncogenic. Cationic lipids for gene delivery represent an attractive alternative to viral vectors for cell transfection in vitro and in vivo. Studies of structure-activity relationships and mechanism governing the formation and behaviour of cationic liposomes in gene delivery were important in improving transfection efficiency and optimizing gene therapy method. The cellular pathways and steps leading to gene transfection for cationic reagents were reviewed. Formation of the cationic lipid, DNA complexs, entry of cationic lipid: DNA complexs into cells, escape from endosomes and dissociation of the complexes and nuclear entry will also be addressed. The plasmid