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8 9 6 ICGC NORTH AMERICA VOLUME 22 NUMBER 9 SEPTEMBER ZOtM www.
The Evolution of Capillary
Gel Electrophoresis: From
Proteins to DNA Sequencing
Chromatography lectrophoresis (from the Greek induced fluorescence and electrochemical
E words elektron : electron; and detection opened new, previously incon-
phoresis: carrying) is a separation ceivable detection limits as low as in the
method in which charged particles migrate zeptomole (10-^ mol) range. As capillary
under the influence of an electric field at gel electrophoresis separates complex sam-
various rates, depending upon their charge- ple mixtures in just minutes with excellent
to-mass ratios. Electrophoresis as an analyt- teproducibility, even in multicapillary for-
ical method was Introduced by Arnie mat, it is becoming an important separa-
Tiselius in the 1930s to separate human tion and characterization tool in the
Guest Author serum into albumin, a-globulin, (3-glabu- genomics and ptoteomics era.
Andras Guttman
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