第七章植物基因工程new.ppt

第七章 植物基因工程; 转基因植物(Transgene plant)是拥有来自其他物种基因的植物。该基因变化过程可以来自不同物种之间的杂交，但现在该名词更多的特指那些在实验室里通过重组DNA技术人工插入其他物种基因以创造出拥有新特性的植物。 ? ?? ??;;第一节 植物基因的克隆与分离;二、根据mRNA特异分离目的基因;;Neurospheres (NS) are cultured by the usual methods. Sister cultures are differentiated for 24 hours and each are subjected to representational difference analysis (RDA) with two rounds of subtraction. The subtracted products are then cloned into plasmids, propagated in bacteria, and arrayed at high density onto a glass slide. The custom microarray is then screened with amplified complementary DNA derived from a different set