急性早幼粒白血病细胞培养上清液对人脐带血单个核细胞分化的影响.docVIP

精品论文 参考文献 急性早幼粒白血病细胞培养上清液对人脐带血单个核细胞分化的影响 郑毅1,2 方宁1 陈代雄1 (1遵义医学院附属医院/贵州省重点细胞工程实验室 贵州遵义 563003) (2遵义医药高等专科学校生理教研室 贵州遵义 563000) 【摘要】目的 白血病细胞能够通过自分泌方式分泌细胞因子而作用于自身，使细胞大量的增殖而不分化；白血病细胞所分泌的细胞因子也能作用于人脐带血(UCB)单个核细胞(MNCs)中的CD34+/CD133+细胞亚群使之增殖而抑制其分化。关于急性早幼粒白血病细胞(HL-60细胞)培养上清液能否使UCB- MNCs分化尚未见报道，本实验主要目的是观察HL-60细胞培养上清液对人UCB-MNCs分化的影响。 方法　实验分为①有HL-60细胞培养上清液+StemSpanreg; SFEM组;②StemSpanreg; SFEM组;③HL-60细胞培养上清液+高糖(HG)-DMEM组;④HG-DMEM组;⑤HL-60细胞培养上清液+低糖(LG)-DMEM组;⑥LG-DMEM组。收分离的人UCB-MNCs按2.5times;106/瓶分别接种于6组培养液中,37℃,5%CO2,饱和湿度培养，于培养前和培养12天用流式细胞仪(FCM)分析表型CD34，CD90和CD166的变化。结果 各实验组CD34，CD34/CD90和CD166阳性细胞百分比均较培养前升高。结论 HL-60细胞培养上清液对人UCB-MNCs具有一定的抑制分化作用，其有效生物活性物质有待进一步研究。 【关键词】 HL-60细胞 上清液 单个核细胞 分化 【中图分类号】R39 【文献标识码】A 【文章编号】2095-1752（2013）23-0014-02 Effects of human promyelocytic leukemia HL-60 cell supernatant on differetiation of mononulear cells from human umbilical cord blood ZHENG Yi1,2FANG Nin1,CHENG Dai-xiong1 (1.Key Laboratory of Cell Engineering of Guizhou Province;The Affliliated Hospital of Zunyi Medical College,Guizhou Zunyi 563000,China;2.Department of Physiology of Zunyi Medical And Pharmaceutical College,Guizhou Zunyi 563000,China) 【ABSTRACT】Objective：Leukemic cells can excrete cytokines by an autocrine way, which contribute to its large-scale proliferation, and the cytokines may also contribute to the proliferations of CD34+/CD133+ subpopulation of UCB-MNCs too. To the effect of human promyelocytic leukemia HL-60 cell supernatant on the differentiation of UCB-MNCs, there is not related report so far. The aim of this study is to investigate effects of HL-60 cell supernatant on differentiations of mononuclear cells from human umbilical cord blood. Methods：Six various cultural system are listed as follows: ① HL-60 cell supernatant+ StemSpan#61650;SFEM; ② StemSpan#61650;SFEM; ③ HL-60 cell supernatant+ HG-DMEM; ④ HG-DMEM; ⑤ HL-60 cell supernatant LG-DMEM; ⑥ LG-DMEM. 2.50times;106 of UCB-MNCs per bottle wi