纳米雄黄对皮肤鳞癌A431细胞株抑制增殖及抑制新生血管作用的实验研究Experimental study on inhibition of proliferation and inhibition of neovascularization by nano Realgar on A431 cell line of skin squamous cell carcinoma.pdfVIP

提 要 目的：对皮肤肿瘤进行中医文献复习，并通过检测人皮肤鳞癌 A431 细胞用纳米 雄黄处理后细胞增殖抑制及血管生长调控因子表达情况，探讨纳米雄黄抑制血管生长 抗肿瘤的作用机理。 方法：查询并复习中医文献资料，对皮肤鳞癌 A431 细胞进行体外培养，按实验 分组加药后观察其形态及生长状况变化，并分别用 MTT 法、免疫组织化学法和 RT-PCR 法检测加药后细胞增殖抑制、色素上皮源性因子(PEDF)和 VEGFmRNA 表达 情况，探讨纳米雄黄抑制皮肤鳞癌新生血管生成的作用机理。 结果：纳米雄黄可以抑制人皮肤鳞癌 A431 细胞增殖，且与顺铂联合应用有协同 作用，并发现纳米雄黄抑制 A431 细胞增殖的作用与上调 PEDF 的表达和下调VEGF 的表达以抑制新生血管生成有关。 结论：体外实验表明纳米雄黄可显著抑制人皮肤鳞癌 A431 细胞增殖，并通过抑 制其新生血管生成达到抗肿瘤细胞生长的效果，与顺铂有协同作用。 关键词 纳米雄黄；人皮肤鳞状细胞癌 A431 细胞；肿瘤血管生成；细胞增殖 Experimental Research of Realgar nanoparticles on cutaneous squamous Cell carcinoma in the Inhibition of proliferation and Neovascularization suppression Speciality: Clinical combination Medicine of TCM With Western Medicine Author: Lin Yanyan Tutor: Professor Li Xiurong Abstract Objective:Review the literature of TCM on skin tumor.Detect the cell proliferation and blood vessal growth regulatory factor expression of skin squamous cell carcinoma A431 cells treated with nanometer,then explore the realgar nanoparticles to anti-tumor mechanism of action. Methods:Skin squamous cell carcinoma A431 cells were cultured in vitro.Dose in the experimental groups and observe the changes in its morphology and growth conditions,respectively,using the MTT assay,immunohistochemistry and RT-PCR assay dosing inhibition of cell proliferation,the expression of pigment epithelium-derived factor(PEDF) and VEGFmRNA. Results:Realgar nanoparticles can inhibit human skin squamous cells carcinoma A431 cells proliferation and synergy with cisplatin.Realgar nanoparticles can ru-regulate the expression of PEDF and down the expression of VEGF to inhibit angiogenesis. Conclusion:In vitro experim