纳米雄黄干预肺癌A549细胞对VEGF及HIF-1表达的影响Effect of nano Realgar on the expression of VEGF and HIF-1 in lung cancer A549 cells.pdfVIP

提 要 目的：探讨纳米雄黄对人肺腺癌A549细胞株血管内皮生长因子（VEGF）、缺氧诱 导因子（HIF-1）表达的影响及其抗肿瘤血管生成的机理。 方法：采用细胞培养方法，运用免疫组化法、RT-PCR 技术观察纳米雄黄对人肺 癌 A549 细胞 VEGF、HIF-1 的表达的影响，进而探讨纳米雄黄抗肿瘤血管生成的作用 机理。 结果：实验研究证实，纳米雄黄可以抑制人肺癌 A549 细胞增殖，随药物浓度的 增高作用增强，且与顺铂联合应用有协同作用，初步揭示纳米雄黄抗肿瘤血管生成的 机理可能与下调VEGF、HIF-1的表达有关。 结论：纳米雄黄可以通过抑制肿瘤细胞血管生成等途径实现抗肿瘤作用，与顺铂 联合可产生协同作用。 关键词 纳米雄黄；肿瘤血管生成；人肺腺癌A549细胞；VEGF；HIF-1 Explore realgar nano on human lung cancer A549 cells VEGF, HIF-1 expression and its anti-angiogenic mechanism Speciality: Internal Medicine of TCM Author: Yu Lianyang Tutor: Professor Qi Yuanfu Abstract Objective: To explore realgar nano on human lung adenocarcinoma A549 cell line of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor (HIF-1) expression and its anti-angiogenic mechanism. Methods: Using cell culture methods, the use of immunohistochemical methods, RT-PCR technique to observe realgar nano on human lung cancer A549 cells of VEGF, HIF-1 expression, and thus explore the nanometer realgar anti-angiogenic mechanism of action. Results: The experimental study confirmed that realgar nano can inhibit the proliferation of human lung cancer A549 cells with increased drug concentration, the enhanced role of cisplatin synergy, initially revealed nanometer realgar anti-angiogenic mechanism may be downward adjustment of VEGF, HIF-1 expression. Conclusion：Realgar nano through the inhibition of tumor cell angiogenesis pathway to achieve anti-tumor effect of the synergistic effect in combination with cisplatin. Key words nanometer realgar; tumor angiogenesis; human lung adenocarcinoma A549 cells; VEGF; HIF-1 目 录 引 言